Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

RPTPgamma (801-1147) N Terminal GSTS Tag

  • Product Code: X1666E
  • Size: 20 µg
  • Availability: In Stock In Stock
  • Price (USD): $361

Cat #

X1666E		 Quantity:      

Data Sheet

Product Name

RPTPgamma (801-1147) N Terminal GSTS Tag

Host/Source

E.coli

Product Type

Active Enzyme

Reactivity

Human

Applications

Study of enzyme kinetics, regulation and to dephosphorylate target substances

Size

20 µg

Price (USD)

$361

Background

RPTP gamma, also known as Receptor-type tyrosine-protein phosphatase gamma, R-PTP-gamma or PTPRG is a protein tyrosine phosphatase (PTP) is a candidate tumor suppressor gene since it is located on human chromosome 3p14.2-p21, a region frequently deleted in certain types of renal and lung carcinomas. In situ hybridization analysis reveals that RPTP gamma mRNA is expressed in specific regions of the brain and that the localization of RPTP gamma changes during brain development. RPTP gamma is composed of a putative extracellular domain, a single transmembrane domain, and a cytoplasmic portion with two tandem catalytic tyrosine phosphatase domains. The extracellular domain contains a stretch of 266 amino acids with striking homology to the zinc-containing enzyme carbonic anhydrase (CAH), indicating that RPTP gamma and RPTP beta (HPTP zeta) represent a subfamily of receptor tyrosine phosphatases. RPTP gamma may have a function other than catalysis of hydration of metabolic CO2.

Immunogen

Recombinant enyzme produced in E. coli

Formulation

Provided in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione.

Customer Storage

Enzyme should be stored at -20°C. Enzyme should be kept on ice when dispensing

Target Molecular Weight

65.6 kDa

Database Links:

GenBankNM_002841Human

References

1: Yuki T, Ishihara S, Rumi M, Ortega-Cava Cesar F, Kadowaki Y, Kazumori H, Yuki M, Wada T, Miyaoka Y, Yoshino N, Kinoshita Y. Expression of midkine and receptor-like protein tyrosine phosphatase (RPTP)-beta genes in the rat stomach and the influence of rebamipide.
Aliment Pharmacol Ther. 2003 Jul;18 Suppl 1:106-12.

2: Robles Y, Vivas-Mejia PE, Ortiz-Zuazaga HG, Felix J, Ramos X, Pena de Ortiz S.
Hippocampal gene expression profiling in spatial discrimination learning. Neurobiol Learn Mem. 2003 Jul;80(1):80-95.

3: Hirayama T. Protein tyrosine phosphatase beta, a receptor for Helicobacter pylori vacA toxin.
Keio J Med. 2002 Dec;51 Suppl 2:20-3. Review.

4: Shintani T, Maeda N, Noda M. Receptor-like protein tyrosine phosphatase gamma (RPTPgamma), but not PTPzeta/RPTPbeta, inhibits nerve-growth-factor-induced neurite outgrowth in PC12D cells.
Dev Neurosci. 2001;23(1):55-69.

5: Chilton JK, Stoker AW. Expression of receptor protein tyrosine phosphatases in embryonic chick spinal cord. Mol Cell Neurosci. 2000 Oct;16(4):470-80.

6: Barnea G., Silvennoinen O., Shaanan B., Honegger A.M., Canoll P.D., D'Eustachio P., Morse B., Levy J.B., Laforgia S., Huebner K., Musacchio J.M., Sap J., Schlessinger J.;
Identification of a carbonic anhydrase-like domain in the extracellular region of RPTP gamma defines a new subfamily of receptor tyrosine phosphatases.; Mol. Cell. Biol. 13:1497-1506(1993).

7: Kastury K., Ohta M., Lasota J., Moir D., Dorman T., Laforgia S., Druck T., Huebner K.;
Structure of the human receptor tyrosine phosphatase gamma gene (PTPRG) and relation to the familial RCC t(3;8) chromosome translocation.; Genomics 32:225-235(1996).

8: Krueger N.X., Streuli M., Saito H.; Structural diversity and evolution of human receptor-like protein tyrosine phosphatases.;

9: Kaplan R., Morse B., Huebner K., Croce C., Howk R., Ravera M., Ricca G., Jaye M., Schlessinger J.; Cloning of three human tyrosine phosphatases reveals a multigene family of receptor-linked protein-tyrosine-phosphatases expressed in brain.;
Proc. Natl. Acad. Sci. U.S.A. 87:7000-7004(1990).