Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

PTPbeta (1675-1996) N Terminal GST Tag

  • Product Code: X1665E
  • Size: 20 µg
  • Availability: In Stock In Stock
  • Price (USD): $361

Cat #

X1665E		 Quantity:      

Data Sheet

Product Name

PTPbeta (1675-1996) N Terminal GST Tag

Host/Source

E.coli

Product Type

Active Enzyme

Reactivity

Human

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Size

20 µg

Price (USD)

$361

Background

PTP beta, also known as Receptor-type tyrosine-protein phosphatase beta [Precursor], Protein-tyrosine phosphatase beta,R-PTP-beta, PTPRB or PTPB is a protein tyrosine phosphatase and is overexpressed in glioblastoma tumors. PTP beta plays a n important functional role in tumor cell migration and adhesion. Glioblastomas express at least three splice variants of PTPbeta, including long and short receptor forms. The short form of PTPbeta lacks exon 12, which encodes 860 amino acids located in the extracellular domain. In normal brain tissue and graded astrocytomas the long and short PTPbeta forms have an overlapping expression pattern. U87 stable cell lines overexpressing long or short PTPbeta migrate faster and adhere more robustly than parental U87 cells. The involvement of long and short PTPbeta in glioma tumor cell biology also contributes to the value of PTPbeta as a cancer target.

Immunogen

Recombinant enyzme produced in E. coli

Formulation

Provided in 25 mM Tris-HCl, 75 mM NaCl, pH 8.0, 0.05% Tween, 5 mM DTT and 50% glycerol

Customer Storage

Enzyme should be stored at -20°C. Enzyme should be kept on ice when dispensing

Target Molecular Weight

63.7 kDa

Database Links:

GenBankX54131Human

References

[1] Krueger N.X., Streuli M., Saito H.; Structural diversity and evolution of human receptor-like protein tyrosine phosphatases.; EMBO J. 9:3241-3252(1990).

[2] Brunner RM, Rottengatter K, Horstmann RD, Gelhaus A, Goldammer T, Foerster B, Schwerin M.
Assignment of the protein-tyrosine phosphatase beta gene (PTPRB) to cattle chromosome 5q23q24 by in situ hybridization and somatic cell panel analysis.
Cytogenet Genome Res. 2003;103(1-2):203G.

[3] Cheburkin IuV, Kniazeva TG, Peter S, Kniazev IuP, Karelin MI, Shkol'nik MI, Evtushenko VI, Hanson KP, Ullrich A, Kniazev PG. [Molecular portrait of human kidney carcinomas: the gene expression profiling of protein-tyrosine kinases and tyrosine phosphatases which controlled regulatory signals in the cells] Mol Biol (Mosk). 2002 May-Jun;36(3):480-90. Russian.

[4] Harder KW, Anderson LL, Duncan AM, Jirik FR. The gene for receptor-like protein tyrosine phosphatase (PTPRB) is assigned to chromosome 12q15-->q21. Cytogenet Cell Genet. 1992;61(4):269-70.

[5] Lorente G, Nelson A, Mueller S, Kuo J, Urfer R, Nikolich K, Foehr ED. Functional comparison of long and short splice forms of RPTPbeta: Implications for glioblastoma treatment. Neuro-oncol. 2005 Apr;7(2):154-63.