Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

PTP-PEST (2-300)/PTPN12 N Terminal GST Tag

  • Product Code: X1664E
  • Size: 20 µg
  • Availability: In Stock In Stock
  • Price (USD): $361

Cat #

X1664E		 Quantity:      

Data Sheet

Product Name

PTP-PEST (2-300)/PTPN12 N Terminal GST Tag

Synonyms

Protein-tyrosine phosphatase G1, PTPG1

Host/Source

E.coli

Product Type

Active Enzyme

Reactivity

Human

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Size

20 µg

Price (USD)

$361

Background

PEST also known as Tyrosine-protein phosphatase, non-receptor type 12 (PTPN12), Protein-tyrosine phosphatase G1 or PTPG1 is a protein-tyrosine phosphatase (PTP) involved in regulating the Wiskott-Aldrich syndrome protein (WASp). WASp is tyrosine dephosphorylated by (PTP)-PEST via proline, serine, threonine phosphatase interacting protein (PSTPIP)1 binding. PTP-PEST combined with PSTPIP1 inhibits WASp-driven actin polymerization and synapse formation. PTP-PEST plays a central role in regulating WASp and is absolutely required for WASp contributions to T cell activation.

Immunogen

Recombinant enyzme produced in E. coli

Formulation

Provided in 25 mM Tris-HCl, 75 mM NaCl, pH 8.0, 0.05% Tween, 5 mM DTT and 50% glycerol

Customer Storage

Enzyme should be stored at -20°C. Enzyme should be kept on ice when dispensing

Target Molecular Weight

61,4

Database Links:

GenBankNM_002835Human

References

[1] Takekawa M., Itoh F., Hinoda Y., Arimura Y., Toyota M., Sekiya M., Adachi M., Imai K., Yachi A.; Cloning and characterization of a human cDNA encoding a novel putative cytoplasmic protein-tyrosine-phosphatase.; Biochem. Biophys. Res. Commun. 189:1223-1230(1992).

[2] Yang Q., Co D., Sommercorn J., Tonks N.K.;
Cloning and expression of PTP-PEST. A novel, human, nontransmembrane protein tyrosine phosphatase.; J. Biol. Chem. 268:6622-6628(1993).

[3] Yang Q., Co D., Sommercorn J., Tonks N.K.; pest
J. Biol. Chem. 268:17650-17650(1993).

[4] Schmidt-Arras DE, Bohmer A, Markova B, Choudhary C, Serve H, Bohmer FD. Tyrosine phosphorylation regulates maturation of receptor tyrosine kinases.
Mol Cell Biol. 2005 May;25(9):3690-703.

[5] Wisniewska M, Bossenmaier B, Georges G, Hesse F, Dangl M, Kunkele KP, Ioannidis I, Huber R, Engh RA. The 1.1 A resolution crystal structure of the p130cas SH3 domain and ramifications for ligand selectivity. J Mol Biol. 2005 Apr 15;347(5):1005-14.

[6] Badour K, Zhang J, Shi F, Leng Y, Collins M, Siminovitch KA. Fyn and PTP-PEST-mediated regulation of Wiskott-Aldrich syndrome protein (WASp) tyrosine phosphorylation is required for coupling T cell antigen receptor engagement to WASp effector function and T cell activation.
J Exp Med. 2004 Jan 5;199(1):99-112.