Exalpha Biologicals, Inc.

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Nordic-MUbio develops and manufactures high quality antibody reagents for use in R&D. All our products are manufactured according to strict ISO 9001 quality guidelines, and our focus is on developing antibodies targetting Cell Adhesion, Nuclear and Cytoskeletal Proteins.

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Exalpha Biologicals, Inc.

PTP-PEST (2-300)/PTPN12 N Terminal GST Tag

  • Product Code: X1664E
  • Size: 20 µg
  • Availability: In Stock In Stock
  • Price (USD): $361

Cat #

X1664E		 Quantity:      

Data Sheet

Product Name

PTP-PEST (2-300)/PTPN12 N Terminal GST Tag

Synonyms

Protein-tyrosine phosphatase G1, PTPG1

Host/Source

E.coli

Product Type

Active Enzyme

Reactivity

Human

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Size

20 µg

Price (USD)

$361

Background

PEST also known as Tyrosine-protein phosphatase, non-receptor type 12 (PTPN12), Protein-tyrosine phosphatase G1 or PTPG1 is a protein-tyrosine phosphatase (PTP) involved in regulating the Wiskott-Aldrich syndrome protein (WASp). WASp is tyrosine dephosphorylated by (PTP)-PEST via proline, serine, threonine phosphatase interacting protein (PSTPIP)1 binding. PTP-PEST combined with PSTPIP1 inhibits WASp-driven actin polymerization and synapse formation. PTP-PEST plays a central role in regulating WASp and is absolutely required for WASp contributions to T cell activation.

Immunogen

Recombinant enyzme produced in E. coli

Formulation

Provided in 25 mM Tris-HCl, 75 mM NaCl, pH 8.0, 0.05% Tween, 5 mM DTT and 50% glycerol

Customer Storage

Enzyme should be stored at -20°C. Enzyme should be kept on ice when dispensing

Target Molecular Weight

61,4

Database Links:

GenBankNM_002835Human

References

[1] Takekawa M., Itoh F., Hinoda Y., Arimura Y., Toyota M., Sekiya M., Adachi M., Imai K., Yachi A.; Cloning and characterization of a human cDNA encoding a novel putative cytoplasmic protein-tyrosine-phosphatase.; Biochem. Biophys. Res. Commun. 189:1223-1230(1992).

[2] Yang Q., Co D., Sommercorn J., Tonks N.K.;
Cloning and expression of PTP-PEST. A novel, human, nontransmembrane protein tyrosine phosphatase.; J. Biol. Chem. 268:6622-6628(1993).

[3] Yang Q., Co D., Sommercorn J., Tonks N.K.; pest
J. Biol. Chem. 268:17650-17650(1993).

[4] Schmidt-Arras DE, Bohmer A, Markova B, Choudhary C, Serve H, Bohmer FD. Tyrosine phosphorylation regulates maturation of receptor tyrosine kinases.
Mol Cell Biol. 2005 May;25(9):3690-703.

[5] Wisniewska M, Bossenmaier B, Georges G, Hesse F, Dangl M, Kunkele KP, Ioannidis I, Huber R, Engh RA. The 1.1 A resolution crystal structure of the p130cas SH3 domain and ramifications for ligand selectivity. J Mol Biol. 2005 Apr 15;347(5):1005-14.

[6] Badour K, Zhang J, Shi F, Leng Y, Collins M, Siminovitch KA. Fyn and PTP-PEST-mediated regulation of Wiskott-Aldrich syndrome protein (WASp) tyrosine phosphorylation is required for coupling T cell antigen receptor engagement to WASp effector function and T cell activation.
J Exp Med. 2004 Jan 5;199(1):99-112.