Exalpha Biologicals, Inc.

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Exalpha Biologicals, Inc.

SHP-2 (224-529)/PTPN11

  • Product Code: X1663E
  • Size: 20 µg
  • Availability: In Stock In Stock
  • Price (USD): $361

Cat #

X1663E		 Quantity:      

Data Sheet

Product Name

SHP-2 (224-529)/PTPN11

Host/Source

E.coli

Product Type

Active Enzyme

Reactivity

Human

Applications

Enyzme activity assay standard

Size

20 µg

Price (USD)

$361

Background

SHP-2, also known as Tyrosine-protein phosphatase, non-receptor type 11 (PTPN11), Protein-tyrosine phosphatase 2C, PTP-2C, PTP-1D, SH-PTP3 and SH-PTP2 is protein tyrosine phosphatase which relays signals from growth factor receptors to Ras and other effectors. Germline PTPN11 mutations underlie ~50% of Noonan Syndrome (NS), a developmental disorder associated with an elevated risk of juvenile myelomonocytic leukemia (JMML). Somatic PTPN11 mutations were recently identified in ~35% of JMML patients; these mutations introduce amino acid substitutions that are distinct from those found in NS. These myeloid leukemias include activating mutations as in the RAS family members, and in the receptor tyrosine kinases KIT and FLT3, loss of function of NF-1 mutants, and gain-of-function mutations in the hematopoietic phosphatase SHP-2. Although these mutations collectively account for as many as 50% of cases of AML, with rare exception, only one of these is mutant in any given patient. This epidemiologic observation suggests that these mutations can be viewed as a complementation group and that any one of these is sufficient to contribute proliferative and survival advantage to a leukemic cell.

Immunogen

Recombinant enyzme produced in E. coli

Formulation

Provided in 25 mM Tris-HCl, 75 mM NaCl, pH 8.0, 0.05% Tween, 1 mM DTT and 50% glycerol 2 mM EDTA, 10 mM glutathione.

Customer Storage

Enzyme should be stored at -20°C. Enzyme should be kept on ice when dispensing

Target Molecular Weight

59.5 kDa

Database Links:

GenBankNM_002834Human

References

[1] Ahmad S., Banville D.L., Zhao Z., Fischer E.H., Shen S.H.;
A widely expressed human protein-tyrosine phosphatase containing src homology 2 domains.;
Proc. Natl. Acad. Sci. U.S.A. 90:2197-2201(1993).

[2] Vogel W., Lammers R., Huang J., Ullrich A.;
Activation of a phosphotyrosine phosphatase by tyrosine phosphorylation.; Science 259:1611-1614(1993).

[3] Adachi M., Sekiya M., Miyachi T., Matsuno K., Hinoda Y., Imai K., Yachi A.;
Molecular cloning of a novel protein-tyrosine phosphatase SH-PTP3 with sequence similarity to the src-homology region 2.; FEBS Lett. 314:335-339(1992).

[4] Bastien L., Ramachandran C., Liu S., Adam M.;
Cloning, expression and mutational analysis of SH-PTP2, human protein-tyrosine phosphatase. Biochem. Biophys. Res. Commun. 196:124-133(1993).

[5] Freeman R.M. Jr., Plutzky J., Neel B.G.;
Identification of a human src homology 2-containing protein-tyrosine-phosphatase: a putative homolog of Drosophila corkscrew.; Proc. Natl. Acad. Sci. U.S.A. 89:11239-11243(1992).

[6] Bennett A.M., Tang T.L., Sugimoto S., Walsh C.T., Neel B.G.;
Protein-tyrosine-phosphatase SHPTP2 couples platelet-derived growth factor receptor beta to Ras. Proc. Natl. Acad. Sci. U.S.A. 91:7335-7339(1994).

[7] Salomon A.R., Ficarro S.B., Brill L.M., Brinker A., Phung Q.T., Ericson C., Sauer K., Brock A., Horn D.M., Schultz P.G., Peters E.C.;
Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry.; Proc. Natl. Acad. Sci. U.S.A. 100:443-448(2003).

[8] Kharitonenkov A., Chen Z., Sures I., Wang H., Schilling J., Ullrich A.;
A family of proteins that inhibit signalling through tyrosine kinase receptors.; Nature 386:181-186(1997).

[9] Hof P., Pluskey S., Dhe-Paganon S., Eck M.J., Shoelson S.E.;
Crystal structure of the tyrosine phosphatase SHP-2.; Cell 92:441-450(1998).

[10] Tartaglia M., Mehler E.L., Goldberg R., Zampino G., Brunner H.G., Kremer H., van der Burgt I., Crosby A.H., Ion A., Jeffery S., Kalidas K., Patton M.A., Kucherlapati R.S., Gelb B.D.;
Mutations in PTPN11, encoding the protein tyrosine phosphatase SHP-2, cause Noonan syndrome.; Nat. Genet. 29:465-468(2001).