Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Exalpha Biologicals, Inc.

MEG-2 (285-593)/PTPN9

  • Product Code: X1662E
  • Size: 20 µg
  • Availability: In Stock In Stock
  • Price (USD): $361

Cat #

X1662E		 Quantity:      

Data Sheet

Product Name

MEG-2 (285-593)/PTPN9

Synonyms

Protein-tyrosine phosphatase MEG2, PTPase-MEG2

Host/Source

E.coli

Product Type

Active Enzyme

Reactivity

Human

Applications

Study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Size

20 µg

Price (USD)

$361

Background

MEG-2 also known as PTPN9 is a protein-tyrosine phosphatase originally isolated from peripheral neutrophilic polymorphonuclear leukocytes (PMN). MEG2 is predominantly cytosolic with components present in secondary and tertiary granules and secretory vesicles. MEG2 activity is inhibited after exposure of cells to opsonized zymosan or to phorbol 12-myristate 13-acetate but largely unaffected by the f-met-leu-phe (N-formyl-methionyl-leucyl-phenyalanine). Cysteine 515 is essential for catalytic activity of MEG-2, whereas the noncatalytic (N-terminal) domain of MEG2 negatively regulates the enzymatic activity of the C-terminal phosphatase domain. The activity of MEG2 is enhanced by specific polyphosphoinositides. MEG2 associates at an early stage with nascent phagosomes. MEG2 is a polyphosphoinositide-activated tyrosine phosphatase that may be involved in signaling events regulating phagocytosis, and essential antimicrobial function in the immune response.

Immunogen

Recombinant enyzme produced in E. coli

Formulation

Provided in 25 mM Tris-HCl, pH 8.0, 67 mM NaCl, 50% glycerol, 1.4 mM KCl, 3 mM DTT, 10 mM glutathione.

Customer Storage

Enzyme should be stored at -20°C. Enzyme should be kept on ice when dispensing

Target Molecular Weight

60.3 kDa

Database Links:

GenBankM83738Human

References

1: Huynh H, Bottini N, Williams S, Cherepanov V, Musumeci L, Saito K, Bruckner S, Vachon E, Wang X, Kruger J, Chow CW, Pellecchia M, Monosov E, Greer PA, Trimble W, Downey GP, Mustelin T.
Control of vesicle fusion by a tyrosine phosphatase. Nat Cell Biol. 2004 Sep;6(9):831-9. Epub 2004 Aug 22.

2:Huynh H, Wang X, Li W, Bottini N, Williams S, Nika K, Ishihara H, Godzik A, Mustelin T.
Homotypic secretory vesicle fusion induced by the protein tyrosine phosphatase MEG2 depends on polyphosphoinositides in T cells. J Immunol. 2003 Dec 15;171(12):6661-71.

3: Xu MJ, Sui X, Zhao R, Dai C, Krantz SB, Zhao ZJ.
PTP-MEG2 is activated in polycythemia vera erythroid progenitor cells and is required for growth and expansion of erythroid cells. Blood. 2003 Dec 15;102(13):4354-60. Epub 2003 Aug 14.

4: Zhao R, Fu X, Li Q, Krantz SB, Zhao ZJ.
Specific interaction of protein tyrosine phosphatase-MEG2 with phosphatidylserine. J Biol Chem. 2003 Jun 20;278(25):22609-14. Epub 2003 Apr 17.

5: Qi Y, Zhao R, Cao H, Sui X, Krantz SB, Zhao ZJ.
Purification and characterization of protein tyrosine phosphatase PTP-MEG2.
J Cell Biochem. 2002;86(1):79-89.

6: Wang X, Huynh H, Gjorloff-Wingren A, Monosov E, Stridsberg M, Fukuda M, Mustelin T.
Enlargement of secretory vesicles by protein tyrosine phosphatase PTP-MEG2 in rat basophilic leukemia mast cells and Jurkat T cells.
J Immunol. 2002 May 1;168(9):4612-9.

7: Kruger JM, Fukushima T, Cherepanov V, Borregaard N, Loeve C, Shek C, Sharma K, Tanswell AK, Chow CW, Downey GP. Protein-tyrosine phosphatase MEG2 is expressed by human neutrophils. Localization to the phagosome and activation by polyphosphoinositides.
J Biol Chem. 2002 Jan 25;277(4):2620-8. Epub 2001 Nov 15.