Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

MEG-1 (637-926)/PTPN4 N-Terminal GST Tag

  • Product Code: X1661E
  • Size: 20 µg
  • Availability: In Stock In Stock
  • Price (USD): $361

Cat #

X1661E		 Quantity:      

Data Sheet

Product Name

MEG-1 (637-926)/PTPN4 N-Terminal GST Tag

Synonyms

PP4R1, PPP4R1

Host/Source

E.coli

Product Type

Active Enzyme

Reactivity

Human

Applications

Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.

Size

20 µg

Price (USD)

$361

Background

MEG-1, also know as Serine/threonine phosphatase 4 regulatory subunit 1, PPP4R1 or PP4R1 is crucial to cellular function. The catalytic subunit of MEG-1 or protein phosphatase 4 (PP4C) is 65% identical to PP2AC at the amino acid level and has been placed in the type 2A family of phosphatases. MEG-1 has been highly conserved between species sharing 91% amino acid identity between human and Drosophila. MEG-1 is predominantly localized in the nucleus in rat brain and liver but is most highly expressed in testis. MEG-1 is an essential enzyme in the development of Drosophila embryos.

Immunogen

Recombinant enyzme produced in E. coli

Formulation

Provided in 25 mM Tris-HCl, 75 mM NaCl, pH 8.0, 0.05% Tween, 5 mM DTT and 50% glycerol

Customer Storage

Enzyme should be stored at -20°C. Enzyme should be kept on ice when dispensing

Target Molecular Weight

60.3 kDa

Database Links:

GenBankNM_002830Human

References

[1] Kloeker S., Wadzinski B.E.; Purification and identification of a novel subunit of protein serine/threonine phosphatase 4.; J. Biol. Chem. 274:5339-5347(1999).
[2] Wada T., Miyata T., Inagi R., Nangaku M., Wagatsuma M., Suzuki D., Wadzinski B.E., Okubo K., Kurokawa K.; Cloning and characterization of a novel subunit of protein serine/threonine phosphatase 4 from mesangial cells.; J. Am. Soc. Nephrol. 12:2601-2608(2001).
[3] Zhang X, Ozawa Y, Lee H, Wen YD, Tan TH, Wadzinski BE, Seto E. Histone deacetylase 3 (HDAC3) activity is regulated by interaction with protein serine/threonine phosphatase 4.
Genes Dev. 2005 Apr 1;19(7):827-39.
[4] Kloeker S, Wadzinski BE. Purification and identification of a novel subunit of protein serine/threonine phosphatase 4. J Biol Chem. 1999 Feb 26;274(9):5339-47.