Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Product Highlight

Mouse anti-M13 phage coat protein g8p

Antibodies recognising M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. The monoclonal antibodies manufactured and supplied by Exalpha react with either the pIII (g3p) or pVIII (g8p) proteins of M13 filamentous bacteriophage. All antibodies are available in a purified format. The antibodies are fully validated and are suitable for a wide range of techniques including:

  • ELISA
  • Flow Cytometry
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation
For more information, click here for our M13 Bacteriophage information page.

News

Two more of our excellent products have been published by PubMed:

Potential actionable targets in appendiceal cancer detected by immunohistochemistry, fluorescent in situ hybridization, and mutational analysis
Borazanci, E., et al., J. Gastrointest. Oncol., 8, 164-172 (2017)
Using Exalpha SPARC Antibody (Cat. No. X1867P)

Molecular mechanism underlying the pharmacological interactions of the protein kinase C-β inhibitor enzastaurin and erlotinib in non-small cell lung cancer cells
Steen, N.V., et al., Am. J. Cancer Res., 7, 816-830 (2017)
Using Exalpha's FITC labeled anti PY20 Antibody (Cat. No. X1017)

Exalpha Biologicals, Inc.

TC-PTP (2-315)/PTPN2

  • Product Code: X1660E
  • Size: 20 µg
  • Price (USD): $361

Cat #

X1660E		 Quantity:      

Data Sheet

Product Name

TC- PTP (2-315)/PTPN2

Synonyms

Protein Tyrosine Phosphatase, Non-receptor Type 2; T-cell Protein Tyrosine Phosphatase

Host/Source

E.coli

Product Type

Active Enzyme

Reactivity

Human

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Size

20 µg

Price (USD)

$361

Background

T-cell protein tyrosine phosphatase (TC-PTP), also known as PTPT and PTPN2, is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. This enzyme has two C-terminal end splice variants with distinctly different subcellular localizations. The shorter 45 kilodalton isoform is exclusively nuclear in resting cells, but redistrubutes to the cytosol upon stimulation with growth factors 1 and cellular stress 2 . The longer 48 kilodalton isoform is exclusively found in the endoplasmic reticulum 3 and seems to have distinctly different physiologic substrates from the smaller isoform. 1, 4 Although found in many cell types and tissues, TC-PTP is particularly prominent in hemopoietic cell types. 5, 6 Knockout mice lacking TC-PTP are born viable but die 3 to 5 weeks after birth of erythropoietic and lymphopoietic deficits 7 , indicating a critical role for TC-PTP in bone marrow maturation. TC-PTP will dephosphorylate a wide range of phosphoproteins, such as p52 Shc 6 and receptors for EGF 1 , Insulin 8 and growth hormone. 6 The recombinant protein lacks the C-terminal 100 amino acids that determine intracellular localization but is fully active. 9

Immunogen

Recombinant enyzme produced in E. coli

Formulation

25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione

Customer Storage

Enzyme should be stored at -20°C. Enzyme should be kept on ice when dispensing

Target Molecular Weight

62.3 kDa

Database Links:

GenBankBC016727Human

References

1. Tiganis, T. et al., 1999, J. Biol. Chem. 274:27768.
2. Lam, M.H. et al., 2001, J. Biol. Chem. 276:37700.
3. Lorenzen, J.A. et al., 1995, J. Cell Biol. 131:631.
4. Tiganis, T. et al., 1998, Mol. Cell. Biol. 18:1622.
5. Cool, D.E. et al., 1989, Proc. Natl. Acad. Sci. USA 86:5257.
6. Pasquali, C. et al., 2003, Mol. Endocrinol. 17:2228.
7. You-Ten, K.E. et al., 1997, J. Exp. Med. 186:683.
8. Galic, S. et al., 2003, Mol. Cell. Biol. 23:2096.
9. Cool, D.E. et al., 1990, Proc. Natl. Acad. Sci. USA 87:7280.