Exalpha Biologicals, Inc.

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Nordic-MUbio develops and manufactures high quality antibody reagents for use in R&D. All our products are manufactured according to strict ISO 9001 quality guidelines, and our focus is on developing antibodies targetting Cell Adhesion, Nuclear and Cytoskeletal Proteins.

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Exalpha Biologicals, Inc.

TC-PTP (2-315)/PTPN2

  • Product Code: X1660E
  • Size: 20 µg
  • Availability: In Stock In Stock
  • Price (USD): $361

Cat #

X1660E		 Quantity:      

Data Sheet

Product Name

TC- PTP (2-315)/PTPN2

Synonyms

Protein Tyrosine Phosphatase, Non-receptor Type 2; T-cell Protein Tyrosine Phosphatase

Host/Source

E.coli

Product Type

Active Enzyme

Reactivity

Human

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Size

20 µg

Price (USD)

$361

Background

T-cell protein tyrosine phosphatase (TC-PTP), also known as PTPT and PTPN2, is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. This enzyme has two C-terminal end splice variants with distinctly different subcellular localizations. The shorter 45 kilodalton isoform is exclusively nuclear in resting cells, but redistrubutes to the cytosol upon stimulation with growth factors 1 and cellular stress 2 . The longer 48 kilodalton isoform is exclusively found in the endoplasmic reticulum 3 and seems to have distinctly different physiologic substrates from the smaller isoform. 1, 4 Although found in many cell types and tissues, TC-PTP is particularly prominent in hemopoietic cell types. 5, 6 Knockout mice lacking TC-PTP are born viable but die 3 to 5 weeks after birth of erythropoietic and lymphopoietic deficits 7 , indicating a critical role for TC-PTP in bone marrow maturation. TC-PTP will dephosphorylate a wide range of phosphoproteins, such as p52 Shc 6 and receptors for EGF 1 , Insulin 8 and growth hormone. 6 The recombinant protein lacks the C-terminal 100 amino acids that determine intracellular localization but is fully active. 9

Immunogen

Recombinant enyzme produced in E. coli

Formulation

25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione

Customer Storage

Enzyme should be stored at -20°C. Enzyme should be kept on ice when dispensing

Target Molecular Weight

62.3 kDa

Database Links:

GenBankBC016727Human

References

1. Tiganis, T. et al., 1999, J. Biol. Chem. 274:27768.
2. Lam, M.H. et al., 2001, J. Biol. Chem. 276:37700.
3. Lorenzen, J.A. et al., 1995, J. Cell Biol. 131:631.
4. Tiganis, T. et al., 1998, Mol. Cell. Biol. 18:1622.
5. Cool, D.E. et al., 1989, Proc. Natl. Acad. Sci. USA 86:5257.
6. Pasquali, C. et al., 2003, Mol. Endocrinol. 17:2228.
7. You-Ten, K.E. et al., 1997, J. Exp. Med. 186:683.
8. Galic, S. et al., 2003, Mol. Cell. Biol. 23:2096.
9. Cool, D.E. et al., 1990, Proc. Natl. Acad. Sci. USA 87:7280.