Exalpha Biologicals, Inc.

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

News

Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

TC-PTP (2-315)/PTPN2

  • Product Code: X1660E
  • Size: 20 µg
  • Availability: In Stock In Stock
  • Price (USD): $361

Cat #

X1660E		 Quantity:      

Data Sheet

Product Name

TC- PTP (2-315)/PTPN2

Synonyms

Protein Tyrosine Phosphatase, Non-receptor Type 2; T-cell Protein Tyrosine Phosphatase

Host/Source

E.coli

Product Type

Active Enzyme

Reactivity

Human

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Size

20 µg

Price (USD)

$361

Background

T-cell protein tyrosine phosphatase (TC-PTP), also known as PTPT and PTPN2, is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. This enzyme has two C-terminal end splice variants with distinctly different subcellular localizations. The shorter 45 kilodalton isoform is exclusively nuclear in resting cells, but redistrubutes to the cytosol upon stimulation with growth factors 1 and cellular stress 2 . The longer 48 kilodalton isoform is exclusively found in the endoplasmic reticulum 3 and seems to have distinctly different physiologic substrates from the smaller isoform. 1, 4 Although found in many cell types and tissues, TC-PTP is particularly prominent in hemopoietic cell types. 5, 6 Knockout mice lacking TC-PTP are born viable but die 3 to 5 weeks after birth of erythropoietic and lymphopoietic deficits 7 , indicating a critical role for TC-PTP in bone marrow maturation. TC-PTP will dephosphorylate a wide range of phosphoproteins, such as p52 Shc 6 and receptors for EGF 1 , Insulin 8 and growth hormone. 6 The recombinant protein lacks the C-terminal 100 amino acids that determine intracellular localization but is fully active. 9

Immunogen

Recombinant enyzme produced in E. coli

Formulation

25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione

Customer Storage

Enzyme should be stored at -20°C. Enzyme should be kept on ice when dispensing

Target Molecular Weight

62.3 kDa

Database Links:

GenBankBC016727Human

References

1. Tiganis, T. et al., 1999, J. Biol. Chem. 274:27768.
2. Lam, M.H. et al., 2001, J. Biol. Chem. 276:37700.
3. Lorenzen, J.A. et al., 1995, J. Cell Biol. 131:631.
4. Tiganis, T. et al., 1998, Mol. Cell. Biol. 18:1622.
5. Cool, D.E. et al., 1989, Proc. Natl. Acad. Sci. USA 86:5257.
6. Pasquali, C. et al., 2003, Mol. Endocrinol. 17:2228.
7. You-Ten, K.E. et al., 1997, J. Exp. Med. 186:683.
8. Galic, S. et al., 2003, Mol. Cell. Biol. 23:2096.
9. Cool, D.E. et al., 1990, Proc. Natl. Acad. Sci. USA 87:7280.