Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Exalpha Biologicals, Inc.

PTP1B (1-321)/PTPN1

  • Product Code: X1659E
  • Size: 20 µg
  • Availability: In Stock In Stock
  • Price (USD): $361

Cat #

X1659E		 Quantity:      

Data Sheet

Product Name

PTP1B (1-321)/PTPN1

Synonyms

Protein Tyrosine Phosphatase 1B

Host/Source

E.coli

Product Type

Active Enzyme

Reactivity

Human

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Size

20 µg

Price (USD)

$361

Background

Tyrosine-protein phosphatase, non-receptor type 1 (PTP1B) also know as Protein-tyrosine phosphatase 1B, PTPN1 is a PTP (protein tyrosine phosphatase) first isolated from human placenta. The protein is unrelated to other known phosphatases but is similar to common leukocyte antigen (CD45) and to LAR a homolog of the neural adhesion molecule NCAM. PTP1B negatively regulates insulin sensitivity by dephosphorylating the insulin receptor. Akt is a ser/thr kinase effector of insulin signaling that phosphorylates substrates at the consensus motif RXRXXS/T. PTP1B contains the Akt phosphorylation motif (RYRDVS50), and PTP1B (but not mutants with substitutions for Ser50) are phosphorylated by Akt. Insulin stimulation also causes a significant increase in phosphorylation of PTP1B.

Immunogen

Recombinant enyzme produced in E. coli

Formulation

25 Mm Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione.

Customer Storage

Enzyme should be stored at -20°C. Enzyme should be kept on ice when dispensing

Target Molecular Weight

63.3 kDa

Database Links:

SwissProtM31724Human

References

[1] Chernoff J., Schievella A.R., Jost C.A., Erikson R.L., Neel B.G.; Cloning of a cDNA for a major human protein-tyrosine-phosphatase.; Proc. Natl. Acad. Sci. U.S.A. 87:2735-2739(1990).
[2] Brown-Shimer S., Johnson K.A., Lawrence J.B., Johnson C., Bruskin A., Green N.R., Hill D.E.;
Molecular cloning and chromosome mapping of the human gene encoding protein phosphotyrosyl phosphatase 1B.; Proc. Natl. Acad. Sci. U.S.A. 87:5148-5152(1990).
[3] Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M., , Rogers J.; The DNA sequence and comparative analysis of human chromosome 20.; Nature 414:865-871(2001).
[4] Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F., Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., Hopkins R.F., Jordan H., Moore T., Max S.I., Wang J., , Marra M.A.;
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.; Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002).
[5] Charbonneau H., Tonks N.K., Kumar S., Diltz C.D., Harrylock M., Cool D.E., Krebs E.G., Fischer E.H., Walsh K.A.; Human placenta protein-tyrosine-phosphatase: amino acid sequence and relationship to a family of receptor-like proteins.; Proc. Natl. Acad. Sci. U.S.A. 86:5252-5256(1989).
[6] Flint A.J., Gebbink M.F.G.B., Franza B.R. Jr., Hill D.E., Tonks N.T.; Multi-site phosphorylation of the protein tyrosine phosphatase, PTP1B: identification of cell cycle regulated and phorbol ester stimulated sites of phosphorylation.; EMBO J. 12:1937-1946(1993).
[7] Frangioni J.V., Beahm P.H., Shifrin V., Jost C.A., Neel B.G.; The nontransmembrane tyrosine phosphatase PTP-1B localizes to the endoplasmic reticulum via its 35 amino acid C-terminal sequence.; Cell 68:545-560(1992).
[8] Ravichandran L.V., Chen H., Li Y., Quon M.J.; Phosphorylation of PTP1B at Ser(50) by Akt impairs its ability to dephosphorylate the insulin receptor.; Mol. Endocrinol. 15:1768-1780(2001).
[9] Barford D., Flint A.J., Tonks N.K.; Crystal structure of human protein tyrosine phosphatase 1B.;
Science 263:1397-1404(1994).
[10] Puius Y.A., Zhao Y., Sullivan M., Lawrence D.S., Almo S.C., Zhang Z.Y.; Identification of a second aryl phosphate-binding site in protein-tyrosine phosphatase 1B: a paradigm for inhibitor design.; Proc. Natl. Acad. Sci. U.S.A. 94:13420-13425(1997).
[11] Pannifer A.D., Flint A.J., Tonks N.K., Barford D.; Visualization of the cysteinyl-phosphate intermediate of a protein-tyrosine phosphatase by X-ray crystallography.; J. Biol. Chem. 273:10454-10462(1998).
[12] Groves M.R., Yao Z.-J., Roller P.P., Burke T.R. Jr., Barford D.; Structural basis for inhibition of the protein tyrosine phosphatase 1B by phosphotyrosine peptide mimetics.; Biochemistry 37:17773-17783(1998).