Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

PRL-1 (2-173) N termnal GST tag

  • Product Code: X1657E
  • Size: 10 µg
  • Availability: In Stock In Stock
  • Price (USD): $361

Cat #

X1657E		 Quantity:      

Data Sheet

Product Name

PRL-1 (2-173) N termnal GST tag

Host/Source

E.coli

Isotype

N/A

Product Type

Active Enzyme

Reactivity

Human

Applications

Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.

Size

10 µg

Price (USD)

$361

Background

The product of the PRL-1 gene (alternate gene names PTP4A1 Synonyms:

Immunogen

Recombinant enyzme produced in E. coli

Formulation

Provided in 25 mM Tris-HCl, 75 mM NaCl, pH 8.0, 0.05% Tween, 5 mM DTT and 50% glycerol

Customer Storage

Enzyme should be stored at -20°C. Enzyme should be kept on ice when dispensing

Target Molecular Weight

45.7 kDa

Database Links:

GenBankBC023975Human

References

[1] Cates C.A., Michael R.L., Stayrook K.R., Harvey K.A., Burke Y.D., Randall S.K., Crowell P.L., Crowell D.N.; Prenylation of oncogenic human PTP(CAAX) protein tyrosine phosphatases.; Cancer Lett. 110:49-55(1996).
[2] Peng Y., Genin A., Spinner N.B., Diamond R.H., Taub R.; The gene encoding human nuclear protein tyrosine phosphatase, PRL-1. Cloning, chromosomal localization, and identification of an intron enhancer.; J. Biol. Chem. 273:17286-17295(1998).
[3] Gjorloff-Wingren A, Saxena M, Han S, Wang X, Alonso A, Renedo M, Oh P, Williams S, Schnitzer J, Mustelin T. Subcellular localization of intracellular protein tyrosine phosphatases in T cells.
Eur J Immunol. 2000 Aug;30(8):2412-21.
[4] Yarovinsky TO, Rickman DW, Diamond RH, Taub R, Hageman GS, Bowes Rickman C.
Expression of the protein tyrosine phosphatase, phosphatase of regenerating liver 1, in the outer segments of primate cone photoreceptors. Brain Res Mol Brain Res. 2000 Apr 14;77(1):95-103.
[5] Carter DA. Expression of a novel rat protein tyrosine phosphatase gene. Biochim Biophys Acta. 1998 Nov 8;1442(2-3):405-8.
[6] Zeng Q, Hong W, Tan YH. Mouse PRL-2 and PRL-3, two potentially prenylated protein tyrosine phosphatases homologous to PRL-1. Biochem Biophys Res Commun. 1998 Mar 17;244(2):421-7.
[7] Cates CA, Michael RL, Stayrook KR, Harvey KA, Burke YD, Randall SK, Crowell PL, Crowell DN Prenylation of oncogenic human PTP(CAAX) protein tyrosine phosphatases. Cancer Lett. 1996 Dec 20;110(1-2):49-55.
[8] Takano S, Fukuyama H, Fukumoto M, Kimura J, Xue JH, Ohashi H, Fujita J. PRL-1, a protein tyrosine phosphatase, is expressed in neurons and oligodendrocytes in the brain and induced in the cerebral cortex following transient forebrain ischemia. Brain Res Mol Brain Res. 1996 Aug;40(1):105-15.