Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery
Exalpha Biologicals, Inc.

Lysophosphatidic Acid Acetyltransferse beta

  • Product Code: X1652P
  • Size: 100 µg
  • Price (USD): $355

Cat #

X1652P		 Quantity:      

Data Sheet

Product Name

Lysophosphatidic Acid Acetyltransferse beta

Synonyms

1-AGP acyltransferase 2, 1-AGPAT 2,1-acylglycerol-3-phosphate O-acyltransferase 2, LPAAT-β, 2

Host/Source

Rabbit

Isotype

IgG

Product Type

Antigen Immunoaffinity Purified Polyclonal

Reactivity

Human

Applications

Western Blot, Enzyme Immunoassay

Purification

Antigen Immunoaffiinity Purification

Size

100 µg

Price (USD)

$355

Background

Lysophospholipid acyltransferases (LPLATs) catalyse the addition of fatty acyl moieties to the glycerol backbone of lysophospholipids . In addition to playing a crutial role in the synthesis of structural membrane components the LPLATs are also implicated in cellular signalling responses for cytokines, growth factors and other agonists. Cellular signalling through the interleukin 1 receptor in human mesangial cells and EL-4 cells proceeds by the activation of lysophosphatidic acid acyltransferase (LPAAT). Activation of LPAAT by interleukin 1 results in the generation of unsaturated phosphatidic acid species, that are crucial to the generation of diacylglycerol and interleukin 1 signalling. LPLATs are also involved in signalling for increased interleukin 2 synthesis through the T cell antigen receptor. Activation of T-cells via anti-CD3 stimulation has been shown to increase incorporation of polyunsaturated fatty acids into phosphatidylcholine via lysophosphatidylcholine acyltransferase. Activation of this enzyme is essential for the sustained activation and translocation of protein kinase C.

Immunogen

Synthetic peptide derived from the LPAAT-β protein

Positive Control

Human brain lysate (Cat. No. X1633C)

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

30.9 kDa

Product Image

Image Legend

Western blot analysis using LPAAT-β antibody on human brain lysate.

Database Links:

SwissProtO15120Human

References

[1] Eberhardt C., Gray P.W., Tjoelker L.W.; Human lysophosphatidic acid acyltransferase. cDNA cloning, expression, and localization to chromosome 9q34.3.; J. Biol. Chem. 272:20299-20305(1997).

[2] Stamps A.C., Elmore M.A., Hill M.E., Kelly K., Makda A.A., Finnen M.J.; A human cDNA sequence with homology to non-mammalian lysophosphatidic acid acyltransferases.; Biochem. J. 326:455-461(1997).

[3] West J., Tompkins C.K., Balantac N., Nudelman E., Meengs B., White T., Bursten S., Coleman J., Kumar A., Singer J.W., Leung D.W.; Cloning and expression of two human lysophosphatidic acid acyltransferase cDNAs that enhance cytokine-induced signaling responses in cells.; DNA Cell Biol. 16:691-701(1997).

[4] Leung D.W., Tompkin C.K., West J.; Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases.

[5] Strausberg R.L., et al Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences.; Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002).

[6] Garg A.; Acquired and inherited lipodystrophies.; N. Engl. J. Med. 350:1220-1234(2004).

[7] Agarwal A.K., Arioglu E., de Almeida S., Akkoc N., Taylor S.I., Bowcock A.M., Barnes R.I., Garg A.; AGPAT2 is mutated in congenital generalized lipodystrophy linked to chromosome 9q34.; Nat. Genet. 31:21-23(2002).