Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Product Highlight

Mouse anti-M13 phage coat protein g8p

Antibodies recognising M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. The monoclonal antibodies manufactured and supplied by Exalpha react with either the pIII (g3p) or pVIII (g8p) proteins of M13 filamentous bacteriophage. All antibodies are available in a purified format. The antibodies are fully validated and are suitable for a wide range of techniques including:

  • ELISA
  • Flow Cytometry
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation
For more information, click here for our M13 Bacteriophage information page.

News

Two more of our excellent products have been published by PubMed:

Potential actionable targets in appendiceal cancer detected by immunohistochemistry, fluorescent in situ hybridization, and mutational analysis
Borazanci, E., et al., J. Gastrointest. Oncol., 8, 164-172 (2017)
Using Exalpha SPARC Antibody (Cat. No. X1867P)

Molecular mechanism underlying the pharmacological interactions of the protein kinase C-β inhibitor enzastaurin and erlotinib in non-small cell lung cancer cells
Steen, N.V., et al., Am. J. Cancer Res., 7, 816-830 (2017)
Using Exalpha's FITC labeled anti PY20 Antibody (Cat. No. X1017)

Exalpha Biologicals, Inc.

GPR4

  • Product Code: X1638P
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $371

Cat #

X1638P		 Quantity:      

Data Sheet

Product Name

GPR4

Host/Source

Rabbit

Isotype

IgG

Product Type

Antigen Immunoaffinity Purified Polyclonal

Reactivity

Human

Applications

Western Blot

Purification

Antigen Immunoaffiinity Purification

Size

100 µg

Price (USD)

$371

Background

Sphingosylphosphorylcholine (SPC) and lysophosphatidylcholine (LPC) are bioactive lipid molecules involved in numerous biologicals processes. GPR4 shares sequence homology with OGR1 (51%), the highest of all GPCR’s. GPR4 has been identified as another high affinity receptor for SPC and low affinity receptor for LPC. SPC and LPC stimulate kinase activation and DNA synthesis stimulated, both of which are pertussis toxin-sensitive, suggesting Gi-heterotrimeric G protein involvement. The GPR4 subfamily of GPCR’s consists of four receptors that share significant sequence homology; OGR1, TDAG8 and G2A. G2A has been shown to be a potent transforming oncogene. GPR4 also malignantly transforms NIH3T3 cells and TDAG8 malignantly transforms the normal mammary epithelial cell line NMuMG. Overexpression of GPR4 or TDGA8 in HEK293 cells leads to transcriptional activation independent of exogenously added ligand. TDAG8 and GPR4 are also overexpressed in a range of human cancer tissues suggestive of a driving role in maintaining tumor formation.

Immunogen

Synthetic peptide derived from the GPR4 protein.

Positive Control

Human brain lysate (Cat. No. X1633C)

Formulation

Provided as ligand affinity purified antibody in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Database Links:

SwissProtP46093Human

References

1. Sin, W.C., et al. 'G protein-coupled receptors GPR4 and TDAG8 are oncogenic and overexpressed in human cancers.' Oncogene 2004

2. Yun, M.R., et al. 'The action mode of lysophosphatidylcholine in human monocytes.' J. Pharmacol. Sci. 2004, 94, 45-50

3. Bektas, M., et al. 'The G protein-coupled receptor GPR4 suppresses ERK activation in a ligand-independent manner.' Biochemistry 2003, 42, 12181-12191

4. Lum, H., et al. 'Inflammatory stress increases receptor for lysophosphatidylcholine in human microvascular endothelial cells.' Am. J. Physiol. Heart Circ. Physiol. 2003, 285, H1786-H1789

5. Xu, Y. 'Sphingosylphosphorylcholine and lysophosphatidylcholine: G protein-coupled receptors and receptor-mediated signal transduction.' Biochim Biophys Acta 2002, 1582, 81-88

6. Zhu, K., et al. 'Sphingosylphosphorylcholine and lysophosphatidylcholine are ligands for the G protein-coupled receptro GPR4.' J. Biol. Chem. 2001, 276, 41325-41335

7. An, S., et al. 'Cloning, sequencing and tissue distribution of two related G protein-coupled receptor candidates expressed prominently in human lung tissue.' FEBS Lett. 1995, 375, 121-124