Catalogue

Background
Sphingosine 1-phosphate (S1P) is a highly bioactive lipid that has a myriad of biological effects both intracellularly as a second messenger and extracellularly by binding to the S1P(1-5)/G-protein-coupled receptors of the endothelial differentiation gene family. Intracellularly, at least two enzymes, sphingosine kinase (1 and 2) and S1P phosphatase, regulate the activity of S1P by governing the phosphorylation of S1P. Regulation of hS1Pase1 with the resultant changes in cellular and secreted S1P has important implications in cell proliferation, angiogenesis and apoptosis.

Source

Immunogen: Synthetic peptide derived from the sphingosine 1-phosphate phosphatase 1 protein.

Product

Product Form: Unconjugated

Formulation: Provided as ligand affinity purified antibody in phosphate buffered saline with 0.08% sodium azide

Purification Method: Ammonium Sulfate Precipitation

Concentration: See vial for concentration

Applications
Antibody can be used for Western blotting (1:200 dilution). Tissue lysate should be used at 10-50 µg/lane. Other applications not yet tested. Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Human brain lysate (Cat. No. X1633C)

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Grey, A., et al. 'Osteoblastic Cells Express Phospholipid Receptors and Phosphatases and Proliferate in Response to Sphingosine 1-Phosphate.' Calcid Tissue Int. 2004 2. Pettus, B.J., et al. 'The sphingosine kinase 1/sphingosine 1-phosphate pathway mediates COX-2 induction and PGE2 production in response to TNF-alpha.' FASEB J. 2003, 17, 1411-1421 3. Johnson, K.R., et al. 'Role of human sphingosine 1-phosphate phosphatase 1 in the regulation of intra- and extracellular sphingosine 1-phosphate levels and cell viability.' J. Biol. Chem. 2003, 278, 34541-34547 4. Cuvillier, O. 'Sphingosine in apoptosis signalling.' Biochim. Biophys. Acta 2002, 1585, 153-162 5. Ogawa, C., et al. 'Identification and characterization of a novel human sphingosine 1-phosphate phosphohydrolase, hSPP2.' J. Biol. Chem. 2003, 278, 1268-1272 6. Le Stunff, H., et al. 'Characterization of murine sphingosine 1-phosphate phosphohydrolase.' J. Biol. Chem. 2002, 277, 8920-8927 7. Birchwood, C.J., et al. 'Calcium influx and signaling in yeast stimulated by intracellular sphingosine 1-phosphate accumulation.' J. Biol. Chem. 2001, 276, 11712-11718

Protein Reference(s)

Database Name: UniProt

Accession Number: Q9BX95

Species Accession: Human

Safety Datasheet(s) for this product: