Exalpha Biologicals, Inc.

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Exalpha Biologicals, Inc.

Sphingosine 1-Phosphate Lyase 1

  • Product Code: X1631P
  • Size: 100 µg
  • Price (USD): $371

Cat #

X1631P		 Quantity:      

Data Sheet

Product Name

Sphingosine 1-Phosphate Lyase 1

Synonyms

EC 4.1.2.27, SP-lyase, hSPL or Sphingosine-1-phosphate aldolase. Gene name: SGPL1 or KIAA1252

Host/Source

Rabbit

Isotype

IgG

Product Type

Antigen Immunoaffinity Purified Polyclonal

Reactivity

Human, Mouse, Rat, Bovine

Applications

Western Blot

Purification

Antigen Immunoaffiinity Purification

Size

100 µg

Price (USD)

$371

Background

Sphingosine-1-phosphate lyase cleaves phosphorylated sphingoid bases, such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Elevates stress-induced ceramide production and apoptosis. The catalytic activity of sphingosine 1-phosphate lyase converts sphingosine 1-phosphate to phosphoethanolamine and palmitaldehyde using Pyridoxal phosphate as a co-factor. Sphingosine 1-phosphate lyase is a type III membrane protein found in the endoplasmic reticulum. Sphingosine 1-phosphate lyase is found primarily in the liver and kidney. Sphingosine 1-phosphate lyase belongs to the group II decarboxylase family.

Immunogen

Synthetic peptide derived from the sphingosine 1-phosphate lyase 1 protein.

Positive Control

Madin-Darby Bovine Kidney (MDBK) cell lysate (Cat. No. X1007)

Formulation

Provided as ligand affinity purified antibody in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Image

Image Legend

Western blot analysis using S1P lyase 1 antibody at a dilution of 1:400 on MDBK cell lysate (Cat. No. X1007) using Exalpha's mouse anti-rabbit HRP secondary antibody (Cat. No. X1207M) at 1:75,000.

Database Links:

SwissProtQ8CHN6Rat
SwissProtQ8R0X7Mouse
SwissProtO95470Human

References

1. Van Veldhoven P.P., et al; Human sphingosine-1-phosphate lyase: cDNA cloning, functional expression studies and mapping to chromosome 10q22. Biochim. Biophys. Acta 1487:128-134(2000). 2. Reiss U., et al; Sphingosine-phosphate lyase enhances stress-induced ceramide generation and apoptosis. J. Biol. Chem. 279:1281-1290(2004). 3. Nagase T., et al; Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.; DNA Res. 6:337-345(1999). 4. Strausberg R.L., et al; Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002).


Product Specific References


This product has been used in:

1. Katsuma, Susumu, et al, 'Signalling mechanisms in sphingosine 1-phosphate-promoted mesangial cell proliferation ' Genes to Cells 2002, 7, , 1217-1230