Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Exalpha Biologicals, Inc.

alpha2B-Adrenergic Receptor

  • Product Code: X1630M
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $355

Cat #

X1630M		 Quantity:      

Data Sheet

Product Name

alpha2B- Adrenergic Receptor

Host/Source

Mouse

Clone

5G10

Isotype

IgG1

Product Type

Monoclonal Antibody

Reactivity

Rat, Mouse

Applications

Western Blot, Immunohistochemistry

Purification

Protein A/G Chromatography

Size

100 µg

Price (USD)

$355

Background

The rat alpha 2b adrenergic receptor is a G protein coupled receptor which acts through Gi2 and is responsible for Cellular communication and signal transduction. These receptors are linked to pertussis toxin-sensitive G proteins. Agonist binding leads to inhibition of adenylyl cyclase, inhibition of Ca(2+) channels and activation of K(+) channels. Recently, three distinct subtypes of alpha(2)-AR were described, alpha(2A)-AR, alpha(2B)-AR and alpha(2C)-AR. At present, it is unknown which of these alpha(2)-AR subtype(s) may regulate insulin secretion.

Immunogen

Antibody raised to synthetic peptide internal sequence corresponding to the third intracellular loop of rat alpha 2b adrenergic receptor.

Positive Control

MDCK cells transfected with rat alpha2B adrenergic receptors.

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

50 kDa

Product Image

Image Legend

Western blot analysis using alpha2B adrenergic receptor antibody on MDCK cells transfected to produce alpha2B receptor protein.

Database Links:

SwissProtP19328Human

References

1. Ozawa, Y, et al. 'Alpha2-adrenergic receptor subtype alterations in the brainstem in the sudden infant death syndrome.' Early Hum Dev 2003, 75 Suppl, S129-38
2. Yun, J., et al. 'Gene expression profile of neurodegeneration induced by alpha1B-adrenergic receptor overactivity: NMDA/GABAA dysregulation and apoptosis.' Brain 2003, Pt 12, 2667-81
3. Peterhoff, M., et al. 'Inhibition of insulin secretion via distinct signaling pathways in alpha2-adrenoceptor knockout mice.' Eur J Endocrinol 2003, 4, 343-50
4. Paris, A., et al. 'Activation of alpha 2B-adrenoceptors mediates the cardiovascular effects of etomidate.' Anesthesiology 2003, 99, 4, 889-95
5. Brady, AE, et al. 'Spinophilin stabilizes cell surface expression of alpha 2B-adrenergic receptors.' J Biol Chem 2003, 278, 34, 32405-12
6. Savola, JM, et al. 'Fipamezole (JP-1730) is a potent alpha2 adrenergic receptor antagonist that reduces levodopa-induced dyskinesia in the MPTP-lesioned primate model of Parkinson's disease.' Mov Disord 2003, 8, 872-83
7. Sivenius, K, et al. 'A deletion in the alpha2B-adrenergic receptor gene and autonomic nervous function in central obesity.' Obes Res 2003, 8, 962-70
8. Ge, H, et al. 'Constitutive precoupling to G(i) and increased agonist potency in the alpha(2B)-adrenoceptor.' Biochem Biophys Res Commun 2003, 306, 4, 959-65
9. Sykiotis GP, et al. 'The alpha2B adrenergic receptor deletion/insertion polymorphism in morbid obesity.' Clin Auton Res 2003, 3, 203-7
10. Feldman J, Bousquet P. 'Role of cental alpha2B-adrenergic receptors in blood pressure control and hypertension.' J Hypertens 2003, 5, 871-2
11. Willems EW, et al. 'Possible role of alpha-adrenoceptor subtypes in acute migraine therapy.' Cephalalgia 2003, 4, 245-57