Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery
Exalpha Biologicals, Inc.

Sphingosine Kinase 2

  • Product Code: X1628P
  • Size: 100 µg
  • Price (USD): $371

Cat #

X1628P		 Quantity:      

Data Sheet

Product Name

Sphingosine Kinase 2

Synonyms

EC 2.7.1, SK 2, SPK 2, SPHK2, Gene name: SPHK2 or SPHK or SP

Host/Source

Rabbit

Isotype

IgG

Product Type

Antigen Immunoaffinity Purified Polyclonal

Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA

Purification

Antigen Immunoaffiinity Purification

Size

100 µg

Price (USD)

$371

Background

Sphingosine Kinase 2 (Sphk2) catalyzes the phosphorylation of sphingosine to sphingosine 1-phosphate (S1P), an important signaling molecule with intra- and extracellular functions. Inside the cell S1P acts as a signaling molecule like other sphingolipid metabolites like ceramide and sphingosine. S1P has been implicated in regulating cell differentiation, calcium mobilization from intracellular stores, and apoptosis. The cell surface receptors for S1P are the EDG family of G protein-coupled receptors (S1P Receptors). These receptors couple to multiple G proteins (e.g. S1P1 couples to Gi whereas S1P2 and S1P3 couple to Gq, G13 in addition to Gi) and regulate a extremely wide range of cellular events including cell motility, survival, apoptosis, migration and cell-cell interaction. Important roles for S1P have also been reported in regulation of cardiogenesis, vascular maturation, oocyte survival, immune cell trafficking, cells of the neuronal system and bone cells. S1P levels are regulated by the activity of Sphk (Sphk1 and Sphk2).

Immunogen

Synthetic peptide derived from the C-terminal of Sphingosine Kinase 2

Positive Control

Active Recombinant Sphingosine Kinase Enzyme (Cat. No. X1709E)

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

69.2 kDa

Product Image

Image Legend

Western blot analysis using Sphingosine Kinase 2 antibody on 50 ng of recombinant Sphinogsine Kinase 2 enzyme (Cat. No. X1709E). Antibody used at 1:200 dilution. Visualized using Pierce West Femto substrate system. Secondary used at 1:30k dilution. Exposure for 45 seconds.

Database Links:

SwissProtAF245447Human

References

1. Kihara A, Ikeda M, Kariya Y, Lee EY, Lee YM, Igarashi Y, Sphingosine-1-phosphate lyase is involved in the differentiation of F9 embryonal carcinoma cells to primitive endoderm. J. Biol. Chem. 278 (2003) 14578-14585 2. Choi OH, Kim JH, Kinet JP, Calcium mobilization via sphingosine kinase in signalling by the FceRI antigen receptor. Nature 380 (1996) 634-636 3. Olivera A, Kohama T, Edsall L, Nava V, Cuvillier O, Poulton S, Spiegel S, Sphingosine kinase expression increases intracellular sphingosine-1-phosphate and promotes cell growth and survival. J. Cell Biol. 147 (1999) 545-558 4. Chun J, Goetzl EJ, Hla T, Igarashi Y, Lynch KR, Moolenaar W, Pyne S, Tigyi G, International union of pharacology. XXXIV. Lysophospholipid receptor nomenclature. Pharmacol. Rev. 54 (2002) 265-269 5. Hla T, Signaling and biological actions of sphingosine 1-phosphate. Pharmacol. Res. 47 (2003) 401-407 6. Gregorius K, Mouritsen S, Elsner HI, Hydrocoating: a new method for coupling biomolecules to solid phases. J. Immunol. Methods, 181 (1995) 65-73 7. Zhang JH, Chung TDY, Oldenburg KR, A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J. Biomol. Screening, 4 (1999) 67-73 8. Hong Liu, Masako Sugiura, Victor E. Nava, Lisa C. Edsall, Keita Kono, Samantha Poulton, Sheldon Milstien, Takafumi Kohama, and Sarah Spiegel. Molecular Cloning and Functional Characterization of a Novel Mammalian Sphingosine Kinase Type 2 Isoform. J. Biol. Chem., Vol. 275, Issue 26, 19513-19520, June 30, 2000 Product Specific References: 1. Weigert, A., et al. 'Cleavage of sphinosine kinase 2 by caspase-1 provokes its release from apoptotic cells.' Blood, 2010, 115, 3531-3540. 2. Weigert, A., et al. 'Apoptotic cells promote macrophage survival by releasing the antiapoptotic mediator sphingosine-1-phosphate.' Blood, 2006, 108, 1635-1642.