Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery
Exalpha Biologicals, Inc.

Sphingosine Kinase 1

  • Product Code: X1627P
  • Size: 100 µg
  • Price (USD): $371

Cat #

X1627P		 Quantity:      

Data Sheet

Product Name

Sphingosine Kinase 1

Synonyms

EC 2.7.1, SK 1, SPK 1, SPHK1, Gene name: SPHK1 or SPHK or SP

Host/Source

Rabbit

Isotype

IgG

Product Type

Polyclonal Antibody

Reactivity

Human, Rat, Mouse, Bovine

Applications

Western Blot, ELISA

Purification

Antigen Immunoaffiinity Purification

Size

100 µg

Price (USD)

$371

Background

Sphingosine Kinase 1 (Sphk1) catalyzes the phosphorylation of sphingosine to sphingosine 1-phosphate (S1P), an important signaling molecule with intra- and extracellular functions. Inside the cell S1P acts as a signaling molecule like other sphingolipid metabolites like ceramide and sphingosine. S1P has been implicated in regulating cell differentiation, calcium mobilization from intracellular stores, and apoptosis. The cell surface receptors for S1P are the EDG family of G protein-coupled receptors (S1P Receptors). These receptors couple to multiple G proteins (e.g. S1P1 couples to Gi whereas S1P2 and S1P3 couple to Gq, G13 in addition to Gi) and regulate a extremely wide range of cellular events including cell motility, survival, apoptosis, migration and cell-cell interaction. Important roles for S1P have also been reported in regulation of cardiogenesis, vascular maturation, oocyte survival, immune cell trafficking, cells of the neuronal system and bone cells. S1P levels are regulated by the activity of Sphk (Sphk1 and Sphk2).

Immunogen

Synthetic peptide derived from the C-terminal of human Sphingosine Kinase 1

Positive Control

Madin-Darby Bovine Kidney (MDBK) cell lysate (Cat. No. X1007)

Formulation

Provided as ligand affinity purified antibody in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

42.5

Product Image

Image Legend

Western blot analysis using Sphingosine Kinase 1 antibody at a dilution of 1:400 on MDBK cell lysate (Cat. No. X1007) using Exalpha's mouse anti-rabbit HRP secondary antibody (Cat. No. X1207M) at 1:75,000.

Database Links:

SwissProtQ9NYA1Human

References

1. Kihara A, Ikeda M, Kariya Y, Lee EY, Lee YM, Igarashi Y, Sphingosine-1-phosphate lyase is involved in the differentiation of F9 embryonal carcinoma cells to primitive endoderm. J. Biol. Chem. 278 (2003) 14578-14585

2. Choi OH, Kim JH, Kinet JP, Calcium mobilization via sphingosine kinase in signalling by the FceRI antigen receptor. Nature 380 (1996) 634-636

3. Olivera A, Kohama T, Edsall L, Nava V, Cuvillier O, Poulton S, Spiegel S, Sphingosine kinase expression increases intracellular sphingosine-1-phosphate and promotes cell growth and survival. J. Cell Biol. 147 (1999) 545-558

4. Chun J, Goetzl EJ, Hla T, Igarashi Y, Lynch KR, Moolenaar W, Pyne S, Tigyi G, International union of pharacology. XXXIV. Lysophospholipid receptor nomenclature. Pharmacol. Rev. 54 (2002) 265-269

5. Hla T, Signaling and biological actions of sphingosine 1-phosphate. Pharmacol. Res. 47 (2003) 401-407

6. Gregorius K, Mouritsen S, Elsner HI, Hydrocoating: a new method for coupling biomolecules to solid phases. J. Immunol. Methods, 181 (1995) 65-73

7. Zhang JH, Chung TDY, Oldenburg KR, A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J. Biomol. Screening, 4 (1999) 67-73

8. Ohotski, J., et al. Expression of sphingosine 1-phosphate receptor 4 and sphingosine kinase 1 is associated with outcome in oestrogen receptor-negative breast cancer. Br. J. Can. (2012), 106, 1453-1459

Product Specific References:

1. Weigert, A., et al. 'Apoptotic cells promote macrophage survival by releasing the antiapoptotic mediator sphingosine-1-phosphate.' Blood, 2006, 108, 1635-1642.


Product Specific References


This product has been used in:

1. Manikandan, Jayapal, et al, 'Genome-wide gene expression profiling of human mast cells stimulated by IgE or Fc RI-aggregation reveals a complex network of genes involved in inflammatory responses ' BMC Genomics 2006, 7, 210, Online only
2. Yang, Jianfei, et al., 'Sphingosine Kinase 1 Is a Negative Regulator of CD4 Th1 Cells ' The Journal of Immunology 2005, 175, , 6580-6588
3.El-Shewy, H.M., et al. ‘Low-Density Lipoprotein Induced Expression of Connective Tissue Growth Factor via Transactivation of Sphingosine 1-Phosphate Receptors in Mesangial Cells.’ Molecular Endocrinology, May 2012, 26(5): 833-845.