Catalogue

Background
OGR1 (originally identified as GPR68) is a high-affinity receptor for sphingosylphosphorylcholine (SPC). SPC is a member of a class of bioactive lipids which regulate cellular functions. These functions include cell proliferation and growth inhibition, smooth muscle contration and wound healing. SPC also stimulated signalling pathways that induce tyrosine phosphorylation, activate MAP kinases and protein kinase C, modify ion channel activity and increase intracellular Ca2+ concentration. OGR1 is expressed in placenta, lung, liver, spleen, testis, small intestine and peripheral blood leukocytes. It is not found in thymus, ovary, colon, skeletal muscle, kidney or pancreas.

Synonyms: GPR68; Ovarian Cancer G Protein-Coupled Receptor 1

Source
Rabbits were immunized with recombinant protein derived from human OGR1 (GPR68).

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Protein A/G Chromatography

Concentration: See vial for concentration

Applications
Antibody can be used for Western blotting (5-10 µg/ml). Western blot should be visualized using a high sensitivity secondary/substrate system (i.e. Pierce’s SuperSignal West Femto Maximum Sensitivity Substrate). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: OGR1 transfected cell lysate

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Xu, Y., et al. 'Sphingosylphosphorylcholine is a ligand for ovarian cancer G-protein-coupled receptor 1.' Nature Cell Biol., 2, 261-267 (2000) 2. Singh, L.S., et al. ‘Ovarian cancer G protein-coupled receptor 1, a new metastasis suppressor gene in prostate cancer.’ J. Natl. Cancer Inst., 99, 1313-1327 (2007) 3. Yang, M., et al. ‘Expression of and Role for Ovarian Cancer G-protein-coupled Receptor 1 (OGR1) during Osteoclastogenesis’ J. Biol. Chem., 281, 23598-23605 (2006)

Protein Reference(s)

Database Name: SwissProt

Accession Number: Q15743

Species Accession: Human

Safety Datasheet(s) for this product: