Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

OGR1/GPR68

  • Product Code: X1600P
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $438

Cat #

X1600P		 Quantity:      

Data Sheet

Product Name

OGR1/GPR68

Synonyms

GPR68; Ovarian Cancer G Protein-Coupled Receptor 1

Host/Source

Rabbit

Isotype

IgG

Product Type

Polyclonal Antibody

Reactivity

Human

Applications

Western Blot, IHC, Neutralization

Purification

Ammonium Sulfate Precipitation

Size

100 µg

Price (USD)

$438

Background

OGR1 (originally identified as GPR68) is a high-affinity receptor for sphingosylphosphorylcholine (SPC). SPC is a member of a class of bioactive lipids which regulate cellular functions. These functions include cell proliferation and growth inhibition, smooth muscle contration and wound healing. SPC also stimulated signalling pathways that induce tyrosine phosphorylation, activate MAP kinases and protein kinase C, modify ion channel activity and increase intracellular Ca2+ concentration. OGR1 is expressed in placenta, lung, liver, spleen, testis, small intestine and peripheral blood leukocytes. It is not found in thymus, ovary, colon, skeletal muscle, kidney or pancreas.

Immunogen

Recombinant protein derived from human OGR1 (GPR68) protein

Positive Control

OGR1 transfected cell lysate

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Image

Image Legend

Western blot analysis using OGR1 antibody on cells transfected with OGR1 protein at 10 µg/ml. Blot developed using Pierce?s SuperSignal West Femto Maximum Sensitvity Substrate.

Database Links:

SwissProtQ15743Human

References

1. Xu, Y., et al. 'Sphingosylphosphorylcholine is a ligand for ovarian cancer G-protein-coupled receptor 1.' Nature Cell Biol., 2, 261-267 (2000)

2. Singh, L.S., et al. ?Ovarian cancer G protein-coupled receptor 1, a new metastasis suppressor gene in prostate cancer.? J. Natl. Cancer Inst., 99, 1313-1327 (2007)

3. Yang, M., et al. ?Expression of and Role for Ovarian Cancer G-protein-coupled Receptor 1 (OGR1) during Osteoclastogenesis? J. Biol. Chem., 281, 23598-23605 (2006)




Product Specific References


This product has been used in:

1. Iwai, K., et al. ?RGS18 Acts as a Negative Regulator of Osteoclastogenesis by Modulating the Acid-Sensing OGR1/NFAT Signaling Pathway? J. Bone Miner. Res. 22, 1612-1620 (2007) (Neutralization)