Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

Lysophosphatidic Acid 2 (LPA2) Receptor (EDG4) CT

  • Product Code: X1570P
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $438

Cat #

X1570P		 Quantity:      

Data Sheet

Product Name

Lysophosphatidic Acid 2 (LPA2) Receptor (EDG4) CT

Host/Source

Rabbit

Isotype

IgG

Product Type

Polyclonal Antibody

Reactivity

Human

Applications

Western Blot

Purification

Ammonium Sulfate Precipitation

Size

100 µg

Price (USD)

$438

Background

EDG4 belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG4 is lysophospholipid. There are 6 known members of the EDG receptor family and they are implicated in mediating growthrelated effects such as induction of cellular proliferation, alterations in differentiation and survival and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG4 is expressed in testes, ovarian tumor and leukocyte containing tissues.

Immunogen

Antibody raised against a unique synthetic peptide derived from the N-terminal of the human EDG4 protein. Antigen sequence differs in mouse and rat by one amino acid.

Positive Control

RH7777 cells transfected with EDG4 protein

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Image

Image Legend

Western blot analysis using EDG4/LPA2 (NT) antibody at 5 µg/ml on RH7777 cells transfected with EDG4/LPA2 protein in the absense (1) and presense (2) of blocking peptide.

Database Links:

SwissProtQ9HBW0Human

References

1. Goetzl EJ and An, S. Diversity of cellular receptors and functions for the lysophospholipid growth factos lysophosphatidic acid and sphingosine 1-phosphate. FASEB J. 1998, 12, 1589-98.

2. An, S., Goetzl, E.J., Lee, H. Signaling mechanisms and molecular characteristics of G protein-coupled receptors for lysophosphatidic acid and sphingosine 1-phosphate. J. Cell Biochem. Suppl. 1998, 30-31, 147-57.

3. Yun, C. Chris, et al. 'LPA2 receptor mediates mitogenic signals in human colon cancer cells.' Am. J. Physiol Cell Physiol 2005, 289, C2-C11


Product Specific References


This product has been used in:

1. Yun, C. Chris, et al, 'LPA2 receptor mediates mitogenic signals in human colon cancer cells' American Journal of Cell Physiolgy 2005, 289, , C2-C11
2. Shida, D., et a, 'Aberrant expression of lysophosphatidic acid (LPA) receptors in human colorectal cancer' Laboratory Investigation 2004, 84, , 1352-1362