Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery
Exalpha Biologicals, Inc.

Estrogen Receptor

  • Product Code: X1567M
  • Size: 100 µg
  • Price (USD): $433

Cat #

X1567M		 Quantity:      

Data Sheet

Product Name

Estrogen Receptor

Host/Source

Mouse

Clone

TE111.5D11

Isotype

IgG1

Product Type

Monoclonal Antibody

Reactivity

Human, Mouse, Rat, Sheep, Rabbit, Hamster, Cow, Pig, Dog, Chicken

Applications

Western Blotting, Immunoprecipitation, Gel Supershift, Binding blocking, Chromatin Precipitation

Purification

Protein A/G Chromatography

Size

100 µg

Price (USD)

$433

Background

The estrogen receptor (ER) gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains required for transcription activation function, binding to estrogen response element (ERE) constitutive dimerization, binding to heat shock proteins, and ligand recognition. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.

Immunogen

Hybridoma produced by the fusion of splenocytes from mice immunized with the 35 kDa C-terminal fragment (aa 302-595) of human ER expressed in E. coli and mouse myeloma cells.

Positive Control

T47D Cells

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

67

Product Image

Image Legend

Western blot analysis using estrogen receptor antibody on human cells at 1-2 µg/ml.

Database Links:

SwissProtP11474Human

References

1. Abbondanza, C. et. al. 'Characterization and epitope mapping of a new panel of monoclonal antibodies to estradiol receptor.' Steroids, 1993, 58, 4-12