Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

News

Two more of our excellent products have been published by PubMed:

Potential actionable targets in appendiceal cancer detected by immunohistochemistry, fluorescent in situ hybridization, and mutational analysis
Borazanci, E., et al., J. Gastrointest. Oncol., 8, 164-172 (2017)
Using Exalpha SPARC Antibody (Cat. No. X1867P)

Molecular mechanism underlying the pharmacological interactions of the protein kinase C-β inhibitor enzastaurin and erlotinib in non-small cell lung cancer cells
Steen, N.V., et al., Am. J. Cancer Res., 7, 816-830 (2017)
Using Exalpha's FITC labeled anti PY20 Antibody (Cat. No. X1017)

Exalpha Biologicals, Inc.

BrdU (Bromodeoxyuridine)

  • Product Code: X1545K
  • Size: 50 Slides
  • Availability: In Stock In Stock
  • Price (USD): $376

Cat #

X1545K		 Quantity:      

Data Sheet

Product Name

BrdU (Bromodeoxyuridine)

Product Type

Immunohistochemistry Kit

Reactivity

BrdU Incorporated Cells

Applications

Immunohistochemistry (Paraffin & Frozen)

Size

50 Slides

Price (USD)

$376

Background

Exalpha Biologicals BrdU Immunohistochemistry Kit involves incorporation of BrdU into proliferating cells, in vivo or in vitro, and visual staining (dark brown nuclei) of these cells which is achieved using a biotinylated anti- BrdU antibody followed by Streptavidin-HRP Conjugate and DAB (diaminobenzidine) substrate.

Customer Storage

Store kit at -20°C until first use. Store at 4-8°C thereafter.

Product Specific References


This product has been used in:

1. Sato, S., et al, 'Central control of bone remodeling by neuromedin U' Nature Medicine 2007, 13, , 1234-1240