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Exalpha Biologicals, Inc.

Sphingosine 1 Phosphate 4 Receptor (CT) (EDG6)

  • Product Code: X1533M
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $438

Cat #

X1533M		 Quantity:      

Data Sheet

Product Name

Sphingosine 1 Phosphate 4 Receptor (CT) (EDG6)

Synonyms

Endothelial cell differentiation gene 6 C-terminal; Sphingosine-1-Phosphate Receptor-4 (S1P4), (S1P4) (Sphingosine 1-phosphate receptor Edg-6, S1P receptor Edg-6, Endothelial differentiation G-protein coupled receptor 6, S1PR4, EDG6

Host/Source

Mouse

Clone

1

Isotype

IgG1

Product Type

Monoclonal Antibody

Reactivity

Human

Applications

Western Blot, Flow Cytometry

Purification

Protein A/G Chromatography

Size

100 µg

Price (USD)

$438

Background

EDG6 (S1P4) belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG6 (S1P4) is sphingosine-1-phosphate. There are 8 known members of the EDG receptor family and they are implicated in mediating growth related effects such as induction of cellular proliferation, alterations in differentiation and survival and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG6 (S1P4) is expressed in lymphoid and hematopoietic tissue and in the lungs. Recently, the designation of the EDG receptors has been modified to include the ligand for the receptor. Thus, the new designation for the EDG6 (S1P4) receptor is the sphingosine 1-phosphate receptor-4 (S1P4).

Immunogen

Hybridoma produced by the fusion of splenocytes from mice immunized with Unique synthetic peptide derived from the C-terminal of the EDG6 (S1P4) protein and mouse myeloma cells.

Positive Control

RH7777 cells transfected with EDG6 (S1P4) protein (Cat. No. X1311C)

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Image

Image Legend

Western blot analysis using EDG6 (S1P4) monoclonal antibody (X1533M) on cell lysate transfected with EDG6 (S1P4) protein (cat. no. X1311C) at 5 µg/ml. IF on transiently transfected CHO cells. Developed with Cy5 anti-mouse, nuclei are stained with DAPI.

Database Links:

SwissProtO95977Human

References

1. Yamazaki, Y., et al. 'EDG-6 as a putative sphingosine 1-phosphate receptor coupling to Ca(2+) signaling pathway.' Biochem. Biophys. Res. Commun. 2000, 268, 583-589.

2. Van Brocklyn, J.R., et al. 'Sphingosine 1-phosphate is a ligand for the G protein-coupled receptor EDG-6.' Blood 2000, 95, 2624-2629.

3. Takuwa, Y., et al. 'Subtype-specific, differential activities of the EDG family receptors for sphingosine 1-phosphate, a novel lysophospholipid mediator.' Mol. Cell Endocrinol. 2001, 177, 3-11.

4. Ohotski, J., et al. Expression of sphingosine 1-phosphate receptor 4 and sphingosine kinase 1 is associated with outcome in oestrogen receptor-negative breast cancer. Br. J. Can. (2012), 106, 1453-1459