Exalpha Biologicals, Inc.

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Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

News

Two more of our excellent products have been published by PubMed:

Potential actionable targets in appendiceal cancer detected by immunohistochemistry, fluorescent in situ hybridization, and mutational analysis
Borazanci, E., et al., J. Gastrointest. Oncol., 8, 164-172 (2017)
Using Exalpha SPARC Antibody (Cat. No. X1867P)

Molecular mechanism underlying the pharmacological interactions of the protein kinase C-β inhibitor enzastaurin and erlotinib in non-small cell lung cancer cells
Steen, N.V., et al., Am. J. Cancer Res., 7, 816-830 (2017)
Using Exalpha's FITC labeled anti PY20 Antibody (Cat. No. X1017)

Exalpha Biologicals, Inc.

CD15-NA FITC

  • Product Code: X1496M
  • Size: 50 Tests
  • Availability: In Stock In Stock
  • Price (USD): $232

Cat #

X1496M		 Quantity:      

Data Sheet

Product Name

CD15-NA FITC

Synonyms

Non-Agglutinating CD15, FITC Labeled

Host/Source

Mouse

Clone

MCS-1

Isotype

IgG3

Product Type

Single-Color Reagent

Reactivity

Human

Applications

Flow Cytometry

Purification

Affinity Chromatography

Size

50 Tests

Price (USD)

$232

Background

CD15-FITC is a monoclonal antibody (MAb) labelled with fluorescein isothiocyanate (FITC) designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD15 antigen by flow cytometry. The CD15 MAb is applied for the initial evaluation of acute myeloid leukemia (AML) and myelodysplastic syndromes (MDS) together with a panel of other antibodies. Interpretation of results must be made within the context of the patient?s clinical history and other diagnostic tests by a certified professional.

Formulation

Provided as 0.2µm sterile filtered solution in phosphate buffered saline with 0.08% sodium azide.

Customer Storage

Product should be stored at 4-8°C. DO NOT FREEZE

Product Image

Image Legend

Immunofluorescence staining using CD15-FITC IgG3 antibody (Cat. No. X1496M) on human neutrophils.

References

1. Ball ED. M5. CD15 cluster workshop report. In: Schlossman SF, Boumsell L, Gilks W, Harlan JM, Kishimoto T, Morimoto C, Ritz J, et al. editors. Leucocyte typing V. White cell differentiation antigens. Proceedings of Fifth International Workshop and conference held in Boston, USA 3-7 november, 1993. Volume One. Oxford University Press (1995).
2. Bahia DMM, Yamamoto M, Chauffaille MLF, Kimura EYS, Bordin JO, et al. Aberrant phenotypes in acute myeloid leukemia: a high frequency and clinical significance. Haematologica.86: 801-806 (2001)
3. van Gongen JJM, Adriaansen HJ. Immunobiology of leukemia. In Henderson ES, Lister TA, Greaves MF editors. Leukemia. WB Saunders Company (1996)
4. Menéndez P, et al. Comparison between a lyse-and-then-wash method and a lyse-non-wash technique for the enumeration of CD34+ hematopoietic progenitor cells. Cytometry (Comm. Clin. Cytometry) 34: 264-271 (1998)
5. GratamaJW, Menéndez P, Kraan J, Orfao A. Loss of CD34+ hematopoietic progenitor cells due to washing can be reduced by the use of fixative-free erytrocyte lysing reagents. J Immunol. Methods 239: 13-23 (2000)
6. Protection of Laboratory Workers from occupationally acquired infections. Second edition; approved guideline (2001). Villanova PA: National Committee for Clinical Laboratory Standards; Document M29-A2.
7. Procedures for the collection of diagnostic blood specimens by venipuncture- approved standard; Fifth edition (2003). Wayne PA: National Committee for Clinical Laboratory Standards; Document H3-A5.
8. Clinical applications of flow cytometry: Quality assurance and immunophenotyping of lymphocytes; approved guideline (1998). Wayne PA: National Committee for Clinical Laboratory Standards; Document H42-A.
9. Braylan RC, Orfao A, Borowitz MJ, Davis BH. Optimal number of reagents required to evaluate hematolymphoid neoplasias: results of an international consensus meeting. Cytometry 46: 23-7 (2001)
10. Jennings CD, Foon KA. Recent advances in flow cytometry: application to the diagnosis of hematologic malignancy. Blood 90(8): 2863-2892 (1997)
11. Orfao A, Ortuño F, de Santiago M, López A, San Miguel J. Immunophenotyping of acute leucemias and myelodysplastic syndromes. Cytometry Part A 58A: 62-71 (2004)
12. Reichert et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopathol 60:190-208 (1991)