Exalpha Biologicals, Inc.

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Exalpha Biologicals, Inc.

p53 (a.a. 371-380)

  • Product Code: X1493M
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $244

Cat #

X1493M		 Quantity:      

Data Sheet

Product Name

p53 (a.a. 371-380)

Host/Source

Mouse

Clone

HR231

Isotype

IgG2b

Product Type

Monoclonal Antibody

Reactivity

Human, Monkey, Mouse, Rat

Applications

ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry

Purification

Protein A/G Chromatography

Size

100 µg

Price (USD)

$244

Background

p53 is a 53 kDa transcription factor that can inhibit cell cycle progression or induce apoptosis in response to stress or DNA damage. Disruption of the p53 signalling pathway through various mechanisms is the most common alteration in human cancer occuring in over half of all tumors. The p53 protein is short lived and expressed at low levels in normal cells but accumulates and/or is activated in cells that have undergone genotoxic damage or oncogene activation. Many tumor derived and transformed cell lines express elevated levels of mutant p53 protein. Other genes also implicated in the downstream effects as a result of p53 activation are: p21WAF1, GADD45, 14-3-3, bax, Fas/APO1, KILLER/ DR5, Tsp1, IGF-BP3 and others.

Immunogen

Hybridoma produced by the fusion of splenocytes from immunized with full length human p53 protein and mouse myeloma cells.

Positive Control

Breast or colon carcinoma cells

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Image

Image Legend

Western blot analysis using p53 cln HR231 antibody at 1 µg/ml on native H1299 cells (1) and H1299 cells transfected with human p53. Also shown is a graphic representation of the epitope location.

Database Links:

SwissProtQ8BUE4Mouse
SwissProtQ9BRQ8Human

References

1. Zeng, X., et al. UV but not gamma irradiation accelerates p53-induced apoptosis of teratocarcinoma cells by repressing MDM2 transcription. Cancer Res. 2000, 60, 6184-6188

2. Hussain, S.P., et al. p53 tumor suppressor gene: at the crossroads of molecular carcinogenesis, molecular epidemiology and human risk assessment. Ann. N.Y. Acad. Sci. 2000, 919, 79-85

3. Hellin, A.C., et al. Roles of Nuclear Factor-kappaB, p53 and p21/WAF1 in Daunomycin-induced cell cycle arrest and apoptosis. J. Pharmacol. Exp. Ther. 2000, 295, 870-878