Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.


Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

XRCC1 (X-Ray Repair Cross Complementing 1 Protein)

  • Product Code: X1481M
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $433

Cat #

X1481M		 Quantity:      

Data Sheet

Product Name

XRCC1 (X-Ray Repair Cross Complementing 1 Protein)







Product Type

Monoclonal Antibody




Western Blot, Immunohistochemistry (Paraffin Sections)


Protein A/G Chromatography


100 µg

Price (USD)



Recognizes a protein of 85kDa, identified as the X-Ray Repair Cross Complementing (XRCC1). The C-terminal 96 amino acids of XRCC1 are necessary and sufficient for the specific interaction with DNA ligase III of 103kDa. The region of interaction with DNA polymerase beta is located within residues 84-183 in the N-terminal half of the XRCC1 protein. Reportedly, Poly (ADP-ribose) polymerase also interacts with XRCC1. In testis XRCC1 is expressed at high levels. The XRCC1 gene plays a role for the sensitivity of mammalian cell lines toward ionizing irradiation. Cells with a mutation of this gene present with decreased single strand break repair and reduced recombination repair, they show increased double strand breaks, and sister chromatid exchange is increased up to 10-fold. The XRCC1 might serve as a scaffold protein during base excision-repair.


Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant human XRCC1 protein and mouse myeloma Sp2/0 cells.

Positive Control

HT29, LS174T, HeLa cells, normal testis


Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Image

Image Legend

Western blot of HT29 cell lysate using Exalpha?s anti-XRCC1 antibody.

Database Links:



1. Cappelli E; et al. Involvement of XRCC1 and DNA ligase III gene products in DNA base excision repair. Journal of Biological Chemistry, 1997, 272(38):23970-5.

2. Labudova O; et al. The transcription of the XRCC1 gene in the heart of radiation-resistant and radiation-sensitive mice after ionizing irradiation. Pediatric Research, 1997, 41(3):435-9.

3. Nash RA; et al. XRCC1 protein interacts with one of two distinct forms of DNA ligase III. Biochemistry, 1997, 36(17):5207-11.

4. Andersson BS; et al. Nucleotide excision repair genes as determinants of cellular sensitivity to cyclophosphamide analogs. Cancer Chemotherapy and Pharmacology, 1996, 38(5):406-16.

5. Caldecott KW; et al. XRCC1 polypeptide interacts with DNA polymerase beta and possibly poly (ADP-ribose) polymerase, and DNA ligase III is a novel molecular 'nick-sensor' in vitro. Nucleic Acids Research, 1996, 24:4387-94.

6. Kubota Y; et al. Reconstitution of DNA base excision-repair with purified human proteins: interaction between DNA polymerase beta
and the XRCC1 protein. Embo Journal, 1996, 15:6662-70.

7. Walter CA; et al. Xrcc-1 expression during male meiosis in the mouse. Biology of Reproduction, 1996 Sep, 55(3):630-5.

8. Caldecott KW; et al. Characterization of the XRCC1-DNA ligase III complex in vitro and its absence from mutant hamster cells. Nucleic Acids Research, 1995, 23(23):4836-43.

9. Lamerdin JE; Montgomery MA; Stilwagen SA; Scheidecker LK; Tebbs RS; Brookman KW; Thompson LH; Carrano AV. Genomic sequence comparison of the human and mouse XRCC1 DNA repair gene regions. Genomics, 1995, 25(2):547-54.

10. Wei YF; Robins P; Carter K; Caldecott K; Pappin DJ; Yu GL; Wang RP; Shell BK; Nash RA; Schar P; et al. Molecular cloning and expression of human cDNAs encoding a novel DNA ligase IV and DNA ligase III, an enzyme active in DNA repair and recombination. Molecular and Cellular Biology, 1995, 15(6):3206-16.

11. Zhou ZQ; Walter CA. Expression of the DNA repair gene XRCC1 in baboon tissues. Mutation Research, 1995 Nov, 348(3):111-6.

12. Brookman KW; Tebbs RS; Allen SA; Tucker JD; Swiger RR; Lamerdin JE; Carrano AV; Thompson LH. Isolation and characterization of mouse Xrcc-1, a DNA repair gene affecting ligation. Genomics, 1994 Jul 1, 22(1):180-8.

13. Caldecott KW; McKeown CK; Tucker JD; Ljungquist S; Thompson LH. An interaction between the mammalian DNA repair protein XRCC1 and DNA ligase III. Molecular and Cellular Biology, 1994, 14(1):68-76.

14. Caldecott KW; Thompson LH. Partial correction of the single-strand break repair defect in the CHO mutant EM9 by electroporated recombinant XRCC1 protein. Annals of the New York Academy of Sciences, 1994 Jul 29, 726:336-9.

15. Ljungquist S; Kenne K; Olsson L; Sandstrom M. Altered DNA ligase III activity in the CHO EM9 mutant. Mutation Research, 1994, 314(2):177- 86.

16. Shung B; Miyakoshi J; Takebe H. X-ray- induced transcriptional activation of c-myc and XRCC1 genes in ataxia telangiectasia cells. Mutation Research, 1994, 307(1):43-51.

17. Walter CA; Lu J; Bhakta M; Zhou ZQ; Thompson LH; McCarrey JR. Testis and somatic Xrcc-1 DNA repair gene expression. Somatic Cell and Molecular Genetics, 1994, 20(6):451-61.

18. Lehmann AR. Duplicated region of sequence similarity to the human XRCC1 DNA repair gene in the Schizosaccharomyces pombe rad4/cut5 gene. Nucleic Acids Research, 1993, 21(22):5274.