Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Exalpha Biologicals, Inc.

Mismatch Repair Protein 2 (MSH2)

  • Product Code: X1406M
  • Size: 500 µl
  • Availability: In Stock In Stock
  • Price (USD): $433

Cat #

X1406M		 Quantity:      

Data Sheet

Product Name

Mismatch Repair Protein 2 (MSH2)

Host/Source

Mouse

Clone

2MSH01

Isotype

IgG1

Product Type

Monoclonal Antibody

Reactivity

Human

Applications

Immunohistochemistry (Frozen & Paraffin Sections)

Size

500 µl

Price (USD)

$433

Background

Recognizes a ~102kDa identified as MSH2. Germline mutations in human mismatch repair genes (hMSH2, hMSH6, hMLH1, hPMS2) account for majority of the hereditary non-polyposis colorectal carcinoma (HPNCC). CpG dinucleotides in the hMSH2 and hMLH1 genes are hotspots for HNPCC mutations. These mutations cause a mismatch repair deficiency resulting in a mutator phenotype where the replication errors are not repaired. Microsatellites / simple repeatative sequences are prone to this type of replication errors and instability of these microsatellites correlates with the occurance of HPNCC. hMSH2 binds to another MutS homolog protein GTBP to form a heterodimeric complex called hMutSbeta, which binds to insertion/deletion loops in DNA.

Immunogen

Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant human MSH2 protein and mouse myeloma p3-NS1.Ag4-1 cells.

Positive Control

Tonsil tissue

Formulation

Tissue culture supernatant with 0.09% sodium azide.

Customer Storage

Product should be stored at 4-8°C. DO NOT FREEZE

Product Image

Image Legend

Immunohistochemical staining using MSH2 antibody on formalin fixed, paraffin embedded human tonsil tissue.

Database Links:

SwissProtP43246Human

References

1. Fishel R, et al. 'The human mutator gene homolog hMSH2 and its association with hereditary non-polyposis colorectal carcinoma.' Cell 75: 1027-1038 (1993) .

2. Kolodner r D, et al. 'Structure of the human MSH2 locus and analysis of two Muir-Torre kindreds for msh2 mutations.' Genomics 24:516-526 (1994) .

3. Maliaka Y K, et al. 'CpG dinucleotides in the hMSH2 and hMLH1 genes are hotspots for HNPCC mutations.' Hum. Genet. 97: 251-255 (1996) .

4. Palombo F, et al. 'h MutSbate, a heterodimer of hMSH2 and hMSH3, binds to insertion/deletion loops in DNA.' Curr. Biol. 6:1181-1184 (1996) .