Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

News

Two more of our excellent products have been published by PubMed:

Potential actionable targets in appendiceal cancer detected by immunohistochemistry, fluorescent in situ hybridization, and mutational analysis
Using Exalpha SPARC Antibody (Cat. No. X1867P)

Molecular mechanism underlying the pharmacological interactions of the protein kinase C-β inhibitor enzastaurin and erlotinib in non-small cell lung cancer cells
Using Exalpha's FITC labeled anti PY20 Antibody (Cat. No. X1017)

Exalpha Biologicals, Inc.

BrdU Cell Proliferation

  • Product Code: X1327K2
  • Size: 1000 Tests
  • Availability: In Stock In Stock
  • Price (USD): $511

Cat #

X1327K2		 Quantity:      

Data Sheet

Product Name

BrdU Cell Proliferation

Host/Source

N/A

Isotype

N/A

Product Type

Assay Kit

Reactivity

BrdU Incorporated Cells

Applications

ELISA

Size

1000 Tests

Price (USD)

$511

Background

Evaluation of cell cycle progression is essential for investigations in many scientific fields. Measurement of [3H] thymidine incorporation as cells enter S phase has long been the traditional method for the detection of cell proliferation. Subsequent quantification of [3H] thymidine is performed by scintillation counting or autoradiography. This technology is slow, labor intensive and has several limitations including the handling and disposal of radioisotopes and the necessity of expensive equipment. A well-established alternative to [3H] thymidine uptake has been demonstrated by numerous investigators (1-8). In these methods bromodeoxyuridine (BrdU), a thymidine analog replaces [3H] thymidine. BrdU is incorporated, into newly synthesized DNA strands of actively proliferating cells. Following partial denaturation of double stranded DNA, BrdU is detected immunochemically allowing the assessment of the population of cells, which are actively synthesizing DNA. Exalpha Biologicals BrdU Cell Proliferation Assay involves incorporation of BrdU into cells cultured in microtiter plates using the cell layer as the solid phase. The resultant assay is sensitive, rapid, easy to perform and applicable to high sample throughput. In addition to evaluation of cell proliferation, information such as cell number, morphology and analysis of cellular antigens can be obtained from a single culture.

Customer Storage

Store kit at -20°C until first use. Store at 4-8°C thereafter.

References

1. Hawker JR Jr., 'Chemiluminescence-based BrdU ELISA to measure DNA synthesis.' J Immunol Methods. 2003 Mar 1;274(1-2):77-82.

2. Ang, L.P.K., et al. 'Development of a conjunctival epithelial equivalent with improved proliferative properties using a multistep serum-free culture system.' Investigative Ophthalmology & Visual Science, 2004, 45, 1789-1795


Product Specific References


This product has been used in:

1. Chang, V., et al, 'Adenoviral-mediated gene transfer of ectodysplasin-A2 results in induction of apoptosis and cell-cycle arrest in osteosarcoma cell lines.' Cancer Gene Therapy 2007, 14, , 927-933
2. Sekine, C., et al, 'Successful Treatment of Animal Models of Rheumatoid Arthritis with Small-Molecule Cyclin-Dependent Kinase Inhibitors' Journal of Immunology 2008, 180, , 1954-1961
3. Matta, H., et al, 'Activation of alternative NF- B pathway by human herpes virus 8-encoded Fas-associated death domain-like IL-1 -converting enzyme inhibitory protein (vFLIP)' PNAS 2004, 101, , 9399-9404
4. Manov, I., et al. ?Pronounced cancer resistance in a subterranean rodent, the blind mole-rat, Spalax: in vivo and in vitro evidence.? BMC Biol., 2013, 11, 91
5. Sharvit, E., et al. ?Amplified Inhibition of Stellate Cell Activation Pathways by PPAR- , RAR and RXR Agonists.? PLoS One, 2013, 8, e76541
6. Lenarduzzi, M., et al. ?Hemochromatosis Enhances Tumor Progression via Upregulation of Intracellular Iron in Head and Neck Cancer.? PLoS One, 2013, 8, e74075
7. Vishwamitra, D., et al. ?MicroRNA 96 Is a Post-Transcriptional Suppressor of Anaplastic Lymphoma Kinase Expression.? Am. J. Pathol., 2012, 180, 1772-1780
8. Shirin, H., et al. ?Atorvastatin and rosuvastatin do not prevent thioacetamide induced liver cirrhosis in rats.? World J. Gastroenterol., 2013, 19, 241-248
9. Li, L.-D., et al. ?Effect of active immunization against angiotensin II type 1 (AT1) receptor on hypertension & arterial remodelling in spontaneously hypertensive rats (SHR)? Indian J. Med. Res. 2014, 139, 619-624
10, Gutschalk, C.M., et al. ?GM-CSF enhances tumor invasion by elevated MMP-2, -9, and -26 expression? Cancer Med. 2013, 2, 117-129
11. Boonanantanasam, K., et al. ?Enterococcus faecalis Enhances Cell Proliferation through Hydrogen Peroxide-Mediated Epidermal Growth Factor Receptor Activation? Infect. Immun. 2012, 80, 3545-3558