Exalpha Biologicals, Inc.

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

Sphingosine 1 Phosphate Receptor 1 (EDG-1)

  • Product Code: X1273MP
  • Size: 50 Tests
  • Availability: In Stock In Stock
  • Price (USD): $438

Cat #

X1273MP		 Quantity:      

Data Sheet

Product Name

Sphingosine 1 Phosphate Receptor 1 (EDG-1)

Synonyms

Functional Endothelial Cell Differentiation Gene Receptor-1 (S1P) Membrane Preparation

Product Type

Membrane Preparation

Reactivity

N/A

Applications

[35S]GTPgS Labeling

Size

50 Tests

Price (USD)

$438

Background

EDG1 (S1P1) belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG1 (S1P1) is sphingosine-1-phosphate. There are 8 known members of the EDG receptor family and they are implicated in mediating growth related effects such as induction of cellular proliferation, alterations in differentiation and survival and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG1 (S1P1) is expressed in cardiovascular, leukocyte- containing and other tissues.

Formulation

Provided as a sterile solution in 50 mM HEPES containing 2 mM EDTA, 100 mM NaCl and 1 mM MgCl2 (pH 7.5)

Customer Storage

Product should be stored at -70°C. Aliquot to avoid freeze/thaw cycles

References

1. Tigyi, G., et al. 'Pharmacological characterization of phospholipid growth-factor receptors.' Ann N Y Acad Sci. 2000, 905, 34-53

2. Liu, Y., et al., Edg-1, the G protein-coupled receptor for sphingosine-1-phosphate, is essential for vascular maturation. J. Clin. Invest., 2000, 106, 951-961.

3. Nakajima, N., et al., Expression and characterization of edg-1 receptors in rat cardiomyocytes calcium deregulation in response to sphingosine-1-phosphate. Eur. J. Biochem., 2000, 267, 5679-5686.

4. Goetzl, E.J. and An, S., Diversity of cellular receptors and functions for the lysophospholipid growth factors lysophosphatidic acid and sphingosine 1-phosphate. FASEB J. 2000, 12, 1589-1598.

5. Parrill, A.L., et al. 'Identification of Edg1 receptor residues that recognize sphingosine 1-phosphate.' J. Biol. Chem. 2000, 275, 39379-39384.