Exalpha Biologicals, Inc.

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

News

Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

Estrogen Receptor

  • Product Code: X1266M
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $244

Cat #

X1266M		 Quantity:      

Data Sheet

Product Name

Estrogen Receptor

Host/Source

Mouse

Clone

AER311

Isotype

IgG2a

Product Type

Monoclonal Antibody

Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation, Immunohistochemistry,Enzyme Immunoassay

Purification

Protein A/G Chromatography

Size

100 µg

Price (USD)

$244

Background

The human estrogen receptor has a molecular weight of 67 kDa and consists of 6 functional domains (domains A-F). Functional mapping of the estrogen receptor has determined a transcriptional promoting activity in the A/B domain. The hormone-binding domain (E domain) is located towards the carboxy terminal, whereas the DNA-binding domain (C domain) is found in the central portion of the molecule. It has been speculated that the presence in breast cancer cells of truncated forms of the estrogen receptor lacking the hormone-binding domain might promote the uncontrolled growth of the tumor.

Immunogen

Hybridoma produced by the fusion of splenocytes from mice immunized with purified estrogen receptors from calf uterus and mouse myeloma cells.

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

67

Database Links:

SwissProtP19785Mouse
SwissProtP03372Human

References

1. Abbondanza, E., et al. 'Characterization and epitope mapping of a new panel of monoclonal antibodies to estradiol receptor.' Steroids 1993, 58, 4-12.

2. Santeusanio, G., et al. 'Immunohistochemical analysis of estrogen receptors in breast carcinomas using monoclonal antibodies that recognize different domains of the receptor molecule.' Appl. Immunohistochem Mol Morphol. 2000, 8, 275-284.

3. Hori, M., et al. 'Determination of estrogen receptor in primary breast cancer using two different monoclonal antibodies, and correlation with its mRNA expression.' Pathol. Int. 1999, 49, 191-197.

4. Schuler, G., et al. 'Occurrence of estrogen receptor alpha in bovine placentomes throughout mid and late gestation and at parturition.' Biol. Reprod. 2002, 66, 976-982.


Product Specific References


This product has been used in:

1. Schuler, G., et al. ?Occurrence of estrogen receptor alpha in bovine placentomes throughout mid and late gestation and at parturition.? Biol. Reprod. (2012), 66, 976-982
2. Santeusanio, G., et al. ?Immunohistochemical analysis of estrogen receptors in breast carcinomas using monoclonal antibodies that recognize different domains of the receptor molecule.? Appl. Immunohistochem. Mol. Morphol. (2000), 8, 275-284
3. Hori, M., et al. ?Determination of estrogen receptor in primary breast cancer using two different monoclonal antibodies, and correlation with its mRNA expression.? Pathol. Int. (1999), 49, 191-197
4. Huang, A., et al. ?Immunohistochemical assay for oestrogen receptors in paraffin wax sections of breast carcinoma using a new monoclonal antibody.? J. Pathol. (1996), 180, 223-227