Exalpha Biologicals, Inc.

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Exalpha Biologicals, Inc.

Heat Shock Protein 27

  • Product Code: X1264M
  • Size: 100 µg
  • Price (USD): $249

Cat #

X1264M		 Quantity:      

Data Sheet

Product Name

Heat Shock Protein 27

Host/Source

Mouse

Clone

G3.1

Isotype

IgG1

Product Type

Monoclonal Antibody

Reactivity

Human

Applications

Western Blot,Immunohistochemistry, Enzyme Immunoassay

Purification

Protein A/G Chromatography

Size

100 µg

Price (USD)

$249

Background

HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins (HSP) produced by all organisms studied. HSP27 synthesis is induced by elevated temperature as well as estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosphilia HSP's and mammalian alpha-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore, HSP27 may be useful in classifying various hormone sensitive tumors.

Immunogen

Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with partially purified HSP27 derived from MCF-7 cytosol and mouse myeloma cells.

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Image

Image Legend

Formalin-fixed, paraffin-embedded human breast carcinoma stained with Heat Shock Protein 27 (Cat. #X1264M) using peroxidase-conjugate and DAB chromogen. Note intense cytoplasmic staining of tumor cells.

Database Links:

SwissProtP04792Human

References

1. Adams, D.J., et al. 'Detection of a Mr 24,000 estrogen-regulated protein in human breast cancer by monoclonal antibodies.' Cancer Res. 1983, 43, 4297-4301. 2. Moretti-Rojas, I., et al., 'A cDNA for the estradiol-regulated 24K protein: control of mRNA levels in MCF-7 cells.' Breast Cancer Res. Treat. 1988, 11, 155-163. 3. Hino, M., et al. 'Small heat shock protein 27 (HSP27) associates with tubulin/microtubules in HeLa cells.' Biochem. Biophys. Res. Commun. 2000, 271, 164-169. 4. Ciocca, D.R. and Luque, E.H. 'Immunological evidence for the identity between the hsp27 estrogen-regulated heat shock protein and the p29 estrogen receptor-associated protein in breast and endometrial cancer.' Breast Cancer Res. Treat. 1991, 20, 33-42.