Exalpha Biologicals, Inc.

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

News

Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

Tenascin

  • Product Code: X1261M
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $244

Cat #

X1261M		 Quantity:      

Data Sheet

Product Name

Tenascin

Host/Source

Mouse

Clone

T2H5

Isotype

IgG1

Product Type

Monoclonal Antibody

Reactivity

Human

Applications

Western Blot, Immunohistochemistry (Frozen & Paraffin Sections)

Purification

Protein A/G Chromatography

Size

100 µg

Price (USD)

$244

Background

Tenascin is an extracellular matrix glyoprotein which is found in numerous types of tissues, but most predominately in the nervous system. It has been shown to be upregulated at the edge of healing wounds and in the stroma of many tumors. Three types of tenascin have been identified, tenascin-C, which was the first to be identified, tenascin-R, which is found almost exclusively in the central nervous system, and tenascin-X, a mammalian tenascin found in some connective tissue and around blood vessels.

Immunogen

Hybridoma produced by the fusion of splenocytes from mice immunized with protein preparation from a homogenate of a human mammary tumor and mouse myeloma cells.

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Image

Image Legend

Immunohistochemical staining of human melanoma tissue using Tenascin antibody (Cat. No. X1261M) at 2.5 µg/ml.

Database Links:

SwissProtP24821Human

References

1. Verstraeten, A.A. et al. Tenascin expression in basal cell carcinoma. Br. J. Dermatol. 1992, 127, 571-574

2. Le Poole, I.C., et al. Tenascin is overexpressed in vitiligo lesional skin and inhibits melanocyte adhesion. Br. J. Dermatol. 1997, 137, 171-178

3. Rulo, H.F., et al. Normal human skin demonstrates marked site-variation of tenascin expression, not correlated to epidermal proliferation (Ki-67 binding). J. Dermatol. Sci. 1993, 5, 54-57