Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery
Exalpha Biologicals, Inc.

Leukotactin/Mip-5

  • Product Code: X1242C
  • Size: 20 µg
  • Price (USD): $415

Cat #

X1242C		 Quantity:      

Product Name

Leukotactin/Mip-5

Synonyms

Macrophage Inflammatory Protein-1 delta (MIP-1?), MIP-5, Synthetic Chemokine

Product Type

Synthetic Protein

Reactivity

Human

Applications

Activity Assay

Size

20 µg

Price (USD)

$415

Background

Leukotactin-1 (Lkn-1), also known as Macrophage inflammatory protein 1 delta (MIP-1?), MIP-5, HCC-2, and NCC-3, is a novel human CC chemokine whose gene was mapped to human chromosome 17 adjacent to the HCC-1 gene. Lkn-1, together with mouse C10, mouse MIP-1? and human MPIF-1, constitute a subgroup of CC chemokines which contain six instead of four conserved cysteine residues. The two additional cysteine residues in Lkn-1 have been shown to form a third disulfide bond. Lkn-1 cDNA encodes a 113 amino acid precursor protein with a putative signal peptide of 21 aa residues that is cleaved to generate a 92 amino acid mature protein. In recombinant Lkn-1 preparations produced in insect cells and in yeast, amino-terminal truncations were found to have occurred. The major forms of Lkn-1 secreted by insect cells and yeast were reported to be proteins of 68 and 66 amino acids, respectively. The full length and the amino-terminal truncated forms of human Lkn-1 have been shown to be potent chemoattractants for monocytes and T-lymphocytes. These proteins can also chemoattract eosinophils and have been shown to induce calcium flux in human CCR-1 transfected cells. Additionally, Lkn-1 can suppress colony formation by human granulocyte-macrophage, erythroid, and multipotential progenitor cells stimulated by combinations of growth factors.

Formulation

Lyophilized

Customer Storage

Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

References

1. Zhang, S., et al. Differential effects of leukotactin-1 and macrophage inflammatory protein-1 alpha on neutrophils mediated by CCR1. J. Immunol. 1999, 162, 4938-4942 2. Pardigol, A., et al. HCC-2, a human chemokine: gene structure, expression pattern, and biological activity. Proc. Natl. Acad. Sci. U S A 1998, 95, 6308-6313 3. Youn, B.S., et al. Molecular cloning of leukotactin-1: a novel human beta-chemokine, a chemoattractant for neutrophils, monocytes, and lymphocytes, and a potent agonist at CC chemokine receptors 1 and 3. J. Immunol. 1997, 159, 5201-5205