Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery
Exalpha Biologicals, Inc.

Rap 1A

  • Product Code: X1212
  • Size: 25 µg
  • Price (USD): $283

Cat #

X1212		 Quantity:      

Data Sheet

Product Name

Rap 1A

Synonyms

Rap 1A Recombinant G-Protein, G protein

Host/Source

N/A

Clone

N/A

Isotype

N/A

Product Type

Recombinant Protein

Reactivity

Human

Applications

Affinity Binding

Size

25 µg

Price (USD)

$283

Background

Rap1A is a 21 kDa small GTP-binding protein that antagonizes the Ras transforming activity through tight binding to Ras-GAP. It interferes with Ras-dependent Raf1 activation by inhibiting binding of Ras to the cysteine-rich region of Raf1. This protein is localized in the endoplasmic reticulum, late endosomes, and lysosomes, indicating its potential role in the regulation of intracellular protein degradation. Rap1a contains a C-terminal CLLL motif for post-translational geranylgeranylation and a single site for phosphorylation by protein kinase A. Ras proteins provide a crucial link in the intracellular signalling cascades. Ras proteins are members of the small G protein family. These proteins are characterized by a molecular weight between 20 and 30 kDa, the ability to bind GTP or GDP and a low intrinsic GTPase activity. These proteins relay signals from the plasma membrane activated by receptor tyrosine kinases to the nucleus via a network of serine/threonine kinases. Ras proteins belong to a family of approximately 40 mammalian and 60 non-mammalian proteins. Ras proteins were first discovered as the hyperactive products of mutant Ras genes which promote cancer by disrupting the normal controls on cell proliferation and differentiation; about 30% of human cancers have mutations in a Ras gene.

Immunogen

Recombinant GST protein from E. Coli

Formulation

Provided in 50mM Tris (pH 7.5) solution containing 150 mM NaCl, 10 mM MgCl2, 1 mM DTT, 10% glycerol

Customer Storage

Product should be stored at -70°C. Aliquot to avoid freeze/thaw cycles

Database Links:

SwissProtP62834Human

References

1. Takai, Y., et al. 'Small GTP-binding proteins.' Physiol. Rev. 2001, 81, 153-208

2. McPhee, I., et al. 'Use of an activation-specific probe to show that Rap1A and Rap1B display different sensitivities to activation by forskolin in rat1 cells.' FEBS Lett. 2000, 477, 213-218

3. Herrmann, C., et al. 'Differential interation of the ras family GTP-binding proteins H-Ras, Rap1A and R-Ras with the putative effector molecules Raf kinase and Ral-guanine nucleotide exchange factor.' J. Biol. Chem. 1996, 271, 6794-6800.