Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Product Highlight

Mouse anti-M13 phage coat protein g8p

Antibodies recognising M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. The monoclonal antibodies manufactured and supplied by Exalpha react with either the pIII (g3p) or pVIII (g8p) proteins of M13 filamentous bacteriophage. All antibodies are available in a purified format. The antibodies are fully validated and are suitable for a wide range of techniques including:

  • ELISA
  • Flow Cytometry
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation
For more information, click here for our M13 Bacteriophage information page.

News

Two more of our excellent products have been published by PubMed:

Potential actionable targets in appendiceal cancer detected by immunohistochemistry, fluorescent in situ hybridization, and mutational analysis
Borazanci, E., et al., J. Gastrointest. Oncol., 8, 164-172 (2017)
Using Exalpha SPARC Antibody (Cat. No. X1867P)

Molecular mechanism underlying the pharmacological interactions of the protein kinase C-β inhibitor enzastaurin and erlotinib in non-small cell lung cancer cells
Steen, N.V., et al., Am. J. Cancer Res., 7, 816-830 (2017)
Using Exalpha's FITC labeled anti PY20 Antibody (Cat. No. X1017)

Exalpha Biologicals, Inc.

Rap 1A

  • Product Code: X1212
  • Size: 25 µg
  • Availability: In Stock In Stock
  • Price (USD): $283

Cat #

X1212		 Quantity:      

Data Sheet

Product Name

Rap 1A

Synonyms

Rap 1A Recombinant G-Protein, G protein

Host/Source

N/A

Clone

N/A

Isotype

N/A

Product Type

Recombinant Protein

Reactivity

Human

Applications

Affinity Binding

Size

25 µg

Price (USD)

$283

Background

Rap1A is a 21 kDa small GTP-binding protein that antagonizes the Ras transforming activity through tight binding to Ras-GAP. It interferes with Ras-dependent Raf1 activation by inhibiting binding of Ras to the cysteine-rich region of Raf1. This protein is localized in the endoplasmic reticulum, late endosomes, and lysosomes, indicating its potential role in the regulation of intracellular protein degradation. Rap1a contains a C-terminal CLLL motif for post-translational geranylgeranylation and a single site for phosphorylation by protein kinase A. Ras proteins provide a crucial link in the intracellular signalling cascades. Ras proteins are members of the small G protein family. These proteins are characterized by a molecular weight between 20 and 30 kDa, the ability to bind GTP or GDP and a low intrinsic GTPase activity. These proteins relay signals from the plasma membrane activated by receptor tyrosine kinases to the nucleus via a network of serine/threonine kinases. Ras proteins belong to a family of approximately 40 mammalian and 60 non-mammalian proteins. Ras proteins were first discovered as the hyperactive products of mutant Ras genes which promote cancer by disrupting the normal controls on cell proliferation and differentiation; about 30% of human cancers have mutations in a Ras gene.

Immunogen

Recombinant GST protein from E. Coli

Formulation

Provided in 50mM Tris (pH 7.5) solution containing 150 mM NaCl, 10 mM MgCl2, 1 mM DTT, 10% glycerol

Customer Storage

Product should be stored at -70°C. Aliquot to avoid freeze/thaw cycles

Database Links:

SwissProtP62834Human

References

1. Takai, Y., et al. 'Small GTP-binding proteins.' Physiol. Rev. 2001, 81, 153-208

2. McPhee, I., et al. 'Use of an activation-specific probe to show that Rap1A and Rap1B display different sensitivities to activation by forskolin in rat1 cells.' FEBS Lett. 2000, 477, 213-218

3. Herrmann, C., et al. 'Differential interation of the ras family GTP-binding proteins H-Ras, Rap1A and R-Ras with the putative effector molecules Raf kinase and Ral-guanine nucleotide exchange factor.' J. Biol. Chem. 1996, 271, 6794-6800.