Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Exalpha Biologicals, Inc.

Rap 1A

  • Product Code: X1212
  • Size: 25 µg
  • Availability: In Stock In Stock
  • Price (USD): $283

Cat #

X1212		 Quantity:      

Data Sheet

Product Name

Rap 1A

Synonyms

Rap 1A Recombinant G-Protein, G protein

Host/Source

N/A

Clone

N/A

Isotype

N/A

Product Type

Recombinant Protein

Reactivity

Human

Applications

Affinity Binding

Size

25 µg

Price (USD)

$283

Background

Rap1A is a 21 kDa small GTP-binding protein that antagonizes the Ras transforming activity through tight binding to Ras-GAP. It interferes with Ras-dependent Raf1 activation by inhibiting binding of Ras to the cysteine-rich region of Raf1. This protein is localized in the endoplasmic reticulum, late endosomes, and lysosomes, indicating its potential role in the regulation of intracellular protein degradation. Rap1a contains a C-terminal CLLL motif for post-translational geranylgeranylation and a single site for phosphorylation by protein kinase A. Ras proteins provide a crucial link in the intracellular signalling cascades. Ras proteins are members of the small G protein family. These proteins are characterized by a molecular weight between 20 and 30 kDa, the ability to bind GTP or GDP and a low intrinsic GTPase activity. These proteins relay signals from the plasma membrane activated by receptor tyrosine kinases to the nucleus via a network of serine/threonine kinases. Ras proteins belong to a family of approximately 40 mammalian and 60 non-mammalian proteins. Ras proteins were first discovered as the hyperactive products of mutant Ras genes which promote cancer by disrupting the normal controls on cell proliferation and differentiation; about 30% of human cancers have mutations in a Ras gene.

Immunogen

Recombinant GST protein from E. Coli

Formulation

Provided in 50mM Tris (pH 7.5) solution containing 150 mM NaCl, 10 mM MgCl2, 1 mM DTT, 10% glycerol

Customer Storage

Product should be stored at -70°C. Aliquot to avoid freeze/thaw cycles

Database Links:

SwissProtP62834Human

References

1. Takai, Y., et al. 'Small GTP-binding proteins.' Physiol. Rev. 2001, 81, 153-208

2. McPhee, I., et al. 'Use of an activation-specific probe to show that Rap1A and Rap1B display different sensitivities to activation by forskolin in rat1 cells.' FEBS Lett. 2000, 477, 213-218

3. Herrmann, C., et al. 'Differential interation of the ras family GTP-binding proteins H-Ras, Rap1A and R-Ras with the putative effector molecules Raf kinase and Ral-guanine nucleotide exchange factor.' J. Biol. Chem. 1996, 271, 6794-6800.