Exalpha Biologicals, Inc.

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

Protein Tyrosine Kinase

  • Product Code: X1190E
  • Size: 500 units
  • Availability: In Stock In Stock
  • Price (USD): $244

Cat #

X1190E		 Quantity:      

Data Sheet

Product Name

Protein Tyrosine Kinase

Synonyms

Porcine Protein-Tyrosine Kinase Enzyme

Host/Source

Purified from porcine

Product Type

Active Enzyme

Reactivity

Porcine

Applications

Enzymatic activity standard for use in assays with protein-tyrosine kinases

Size

500 units

Price (USD)

$244

Background

The importance of protein tyrosine phosphorylation in such diverse cellular processes as proliferation, differentiation and gap-juunction communication is reflected in the enormous number of protein tyrosine kinases that have been identified and described in the last thirty years. The roles played by anomalies in expression of these enzymes in cancer and other disease states has made them targets of intense research interest as part of the ongoing search for new therapies. Tyrosine kinases purified from mammalian tissue sources may be valuable to the study of generalized tyrosine phosphorylation or for the generation of specific phosphorylated peptides. Our PTK enzyme has been purified from porcine kidney utilizing both classical chromatographic procedures as well as affinity chromatography on immobilized substrate of random amino acid copolymers containing tyrosine, but not serine or threonine.

Immunogen

Porcine kidney

Formulation

Provided in 10mM Tris (pH 7.4) solution containing 100 mM NaCl, , 50% glycerol

Customer Storage

Product should be stored at -70°C. Aliquot to avoid freeze/thaw cycles

Product Image

Image Legend

Typical time course of phosphorylation comparing PTK enzyme(Cat. No. X1190E) to a commercially available preparation Abl tyrosine kinase.