Exalpha Biologicals, Inc.

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Exalpha Biologicals, Inc.

Farnesyl

  • Product Code: X1165P
  • Size: 100 µg
  • Price (USD): $283

Cat #

X1165P		 Quantity:      

Data Sheet

Product Name

Farnesyl

Host/Source

Rabbit

Isotype

IgG

Product Type

Polyclonal Antibody

Reactivity

Ubiquitous

Applications

IF,Elisa

Purification

Ammonium Sulfate Precipitation

Size

100 µg

Price (USD)

$283

Background

Protein isoprenylation is a post-translational modification that affects about 0.5% of cellular proteins and is essential for the biological activity of proteins. Two enzymes catalyze the attachment of two prenyl groups to the sulfhydryl group of carboxyl-terminal cysteine groups. Proteins which are prenylated by these enzymes have a distinct motif at the C-terminal of the protein, C-A1-A2-X (C = Cysteine, A1 & A2 = aliphatic amino acids). The two enzymes involved in this transfer are farnesyltransferase and geranylgeranyltransferase. These transfer a 15 carbon farnesyl or a 20 carbon geranygeranyl, respectively, from a prenyl-pyrophosphate to the protein. Examples of proteins containing this C-A-A-X motif are members of the Ras small G protein family, the nuclear lamins and the gamma subunit of trimeric G proteins. Prenylation of proteins is necessary for membrane association of proteins as well as protein-protein interactions and the nature of the linked isoprenyl group can influence the protein interactions, such as the interaction between G proteins and receptors.

Immunogen

Antibody developed using farnesyl cysteine conjugated to KLH.

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Image

Image Legend

Immunofluorescence assay using anti-Farnesyl antibody on plasmids encoding isoprenylated protein and visualized using FITC-conjugated goat anti-rabbit antibody.

Database Links:

SwissProtP14324Human

References

1. Baron, R., et al. RhoB prenylation is driven by the three carboxyl-terminal amino acids of the protein: evidenced in vivo by an anti-farnesyl cysteine antibody. Proc. Natl. Acad. Sci. USA 2000, 97, 11626-11631 2. Lin, H.P. et al. Localization of isoprenylated antigen of hepatitis delta virus by anti-farnesyl antibodies. J. Gen. Virol. 1999, 80, 91-96 3. Aspbury, R.A., et al. Isoprenylation of polypeptides in the nematode Caenorhabditis elegans. Biochim Biophys Acta 1998, 1392, 265-275 4. Zhang, F.L. & Casey, P.J. Protein prenylation: molecular mechanisms and functional consequences. Annu. Rev. Biochem. 1996, 65, 241-269 5. Gromov, P.S., et al. Identification of isoprenyl modified proteins metabolically labeled with [3H]farnesyl- and [3H]geranylgeranyl-pyrophosphate. Electrophoresis 1996, 17, 1728-1733


Product Specific References


This product has been used in:

1. Clase, A.C., et al, 'The Pseudorabies Virus Us2 Protein, a Virion Tegument Component, Is Prenylated in Infected Cells' Journal of Virology 2003, 77, , 12285-12298