Exalpha Biologicals, Inc.

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Exalpha Biologicals, Inc.

PIG3 (p53 inducible gene 3)

  • Product Code: X1156M
  • Size: 100 µg
  • Price (USD): $244

Cat #

X1156M		 Quantity:      

Data Sheet

Product Name

PIG3 (p53 inducible gene 3)

Synonyms

Putative quinone oxidoreductase, Tumor protein p53-inducible protein 3, p53-induced gene 3 protein, TP53I3, PIG3

Host/Source

Mouse

Clone

10A2

Isotype

IgG1

Product Type

Monoclonal Antibody

Reactivity

Human

Applications

Western Blot

Purification

Protein A/G Chromatography

Size

100 µg

Price (USD)

$244

Background

p53 inducible gene-3 (PIG-3) may be a long-lived reporter, which may be useful for detecting transient activation of p53. The p53 tumor suppressor is the most commonly mutated gene in human cancers. The p53 protein, which is stabilized in response to different biological checkpoints, is activated by DNA damage, hypoxia, viral infection, or oncogene activation resulting in efects such as cell cycle arrest, apoptosis, senescence, differentiation, and antiangiogenesis. Other genes also implicated in the downstream effects as a result of p53 activation are: p21WAF1, GADD45, 14-3-3, bax, Fas/APO1, KILLER/ DR5, Tsp1, IGF-BP3 and others. The p53-inducible gene 3 (PIG3), was recently identified in a screen for genes induced by p53 before the onset of apoptosis. PIG3 shares significant homology with the oxidoreductases from several species. PIG3 protein is localized to the cytoplasm and induced in primary, non-transformed, and transformed cell cultures after exposure to genotoxic agents. The induction of PIG3 by p53 occurrs with delayed kinetics as compared with other p53 downstream targets, such as p21 and MDM2. PIG3 levels are increased during p53-mediated growth arrest. Elevated levels of PIG3 are maintained in cells that resume cycling in the absence of ectopic p53 expression.

Immunogen

Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant human PIG-3 protein.

Positive Control

Jurkat cell lysate

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Image

Image Legend

Western blot analysis using PIG-3 monoclonal antibody on Jurkat lysate at 10 µg/ml on Jurkat lysate.

Database Links:

SwissProtQORXHuman

References

1. Flatt, P.M., et al., p53-dependent expression of PIG3 during proliferation, genotoxic stress, and reversible growth arrest. Cancer Lett. 2000, 156, 63-73 2. Venot, C., et al., The requirement for the p53 proline-rich functional domain for mediation of apoptosis is correlated with specific PIG3 gene transactivation and with transcriptional repression. EMBO J. 1998, 17, 4668-4679 3. el-Deiry, W.S., Regulation of p53 downstream genes. Semin. Cancer Biol. 1998, 8, 345-357


Product Specific References


This product has been used in:

1. Marusyk, A., et al, 'p53 mediates senescence-like arrest induced by chronic replicational stress' Molecular abd Cell Biology 2007, 27, , 5336-5351