Exalpha Biologicals, Inc.

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

News

Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

PIG3 (p53 inducible gene 3)

  • Product Code: X1155P
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $244

Cat #

X1155P		 Quantity:      

Data Sheet

Product Name

PIG3 (p53 inducible gene 3)

Synonyms

Putative quinone oxidoreductase, Tumor protein p53-inducible protein 3, p53-induced gene 3 protein, TP53I3, PIG3

Host/Source

Rabbit

Isotype

IgG

Product Type

Polyclonal Antibody

Reactivity

Human

Applications

Western Blot

Purification

Ammonium Sulfate Precipitation

Size

100 µg

Price (USD)

$244

Background

p53 inducible gene-3 (PIG-3) may be a long-lived reporter, which may be useful for detecting transient activation of p53. The p53 tumor suppressor is the most commonly mutated gene in human cancers. The p53 protein, which is stabilized in response to different biological checkpoints, is activated by DNA damage, hypoxia, viral infection, or oncogene activation resulting in efects such as cell cycle arrest, apoptosis, senescence, differentiation, and antiangiogenesis. Other genes also implicated in the downstream effects as a result of p53 activation are: p21WAF1, GADD45, 14-3-3, bax, Fas/APO1, KILLER/ DR5, Tsp1, IGF-BP3 and others. The p53-inducible gene 3 (PIG3), was recently identified in a screen for genes induced by p53 before the onset of apoptosis. PIG3 shares significant homology with the oxidoreductases from several species. PIG3 protein is localized to the cytoplasm and induced in primary, non-transformed, and transformed cell cultures after exposure to genotoxic agents. The induction of PIG3 by p53 occurrs with delayed kinetics as compared with other p53 downstream targets, such as p21 and MDM2. PIG3 levels are increased during p53-mediated growth arrest. Elevated levels of PIG3 are maintained in cells that resume cycling in the absence of ectopic p53 expression.

Immunogen

Antibody developed using full-length recombinant PIG-3 protein.

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

35

Product Image

Image Legend

Western blot using anti-PIG3 antibody on RKO cells transfected with pCEP4 without insert (1), transfected with pCEP4-PIG3 (2), under control conditions (3) and treated with adriamycin to activate wild-type p53 (4). Also used on MCF-7 cells transfected with pCEP4 without insert (5) and pCEP4-PIG3 (6).

Database Links:

SwissProtQORXHuman

References

1. Flatt, P.M., et al., p53-dependent expression of PIG3 during proliferation, genotoxic stress, and reversible growth arrest. Cancer Lett. 2000, 156, 63-73

2. Venot, C., et al., The requirement for the p53 proline-rich functional domain for mediation of apoptosis is correlated with specific PIG3 gene transactivation and with transcriptional repression. EMBO J. 1998, 17, 4668-4679

3. el-Deiry, W.S., Regulation of p53 downstream genes. Semin. Cancer Biol. 1998, 8, 345-357


Product Specific References


This product has been used in:

1. Marusyk, A., et al, 'p53 mediates senescence-like arrest induced by chronic replicational stress' Molecular abd Cell Biology 2007, 27, , 5336-5351