Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Exalpha Biologicals, Inc.

Bnip3L (IN) (nix) (bcl 2 family)

  • Product Code: X1120P
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $307

Cat #

X1120P		 Quantity:      

Data Sheet

Product Name

Bnip3L (IN) (nix) (bcl 2 family)

Synonyms

BNIP3A, BNIP3H, NIX

Host/Source

Rabbit

Isotype

IgG

Product Type

Polyclonal Antibody

Reactivity

Human

Applications

Western Blot

Purification

Antigen Immunoaffiinity Purification

Size

100 µg

Price (USD)

$307

Background

Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, Hrk, Nip3, and Bim, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bnip3L, Bnip3 , and Nix (for Nip3-like protein X) (1-3). Bnip3L/Bnip3 /Nix is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3. Overexpression of Bnip3L induces apoptosis (2,3). Bnip3L interacts with and overcomes suppresses by Bcl-2 and Bcl-xL. Bnip3L is localized in mitochondria. The messenger RNA of Bnip3L is ubiquitously expressed in human tissues (1,2). Bnip3L and Bnip3 form a new subfamily of the pro-apoptotic mitochondrial proteins.

Immunogen

Synthetic peptide corresponding to amino acids 77 to 92 of the human Bnip3L protein. Immunogen sequence is identical in the mouse protein.

Positive Control

K562 cell lysate

Formulation

Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

40

Product Image

Image Legend

Western blot analysis using anti-Bnip3L (IN) antibody at 1 µg/ml on K562 whole cell lysate in the absense (1) and presense (2) of immunogenic peptide.

Database Links:

SwissProtO60238Human

References

1. Matsushima, M., et al., Isolation, mapping, and functional analysis of a novel human cDNA (BNIP3L) encoding a protein homologous to human NIP3. Genes Chromosomes Cancer 1998, 21, 230-235

2. Yasuda, M., et al., BNIP3alpha: a human homolog of mitochondrial proapoptotic protein BNIP3. Cancer Res. 1999, 59, 533-537

3. Chen, G., et al., Nix and Nip3 form a subfamily of pro-apoptotic mitochondrial proteins. J. Biol. Chem. 1999, 274, 7-10

4. Imazu, T., et al., Bcl-2/E1B 19 kDa-interacting protein 3-like protein (Bnip3L) interacts with bcl-2/Bcl-xL and induces apoptosis by altering mitochondrial membrane permeability. Oncogene. 1999, 18, 4523-4529

Product Specific References:

1. Aerbajinai, W., et al. 'The proapoptotic factor Nix is coexpressed with Bcl-xL during terminal erythroid differentiation.' Blood, 2003, 102, 712-717.

2. Zhang, J., et al. 'Mitochondrial clearance is regulated by Atg7-dependent and -independent mechanisms during reticulocyte maturation.' Blood, 2009, 114, 157-164.


Product Specific References


This product has been used in:

1. Zhang, J., et al. ?A short linear motif in BNIP3L (NIX) mediates mitochondrial clearance in reticulocytes.? Autophagy, 8, 1325-1332 (2012)