Exalpha Biologicals, Inc.

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

Caspase-12 (NT) (involved in Alzheimers)

  • Product Code: X1115P
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $307

Cat #

X1115P		 Quantity:      

Data Sheet

Product Name

Caspase-12 (NT) (involved in Alzheimers)

Host/Source

Rabbit

Isotype

IgG

Product Type

Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purification

Antigen Immunoaffiinity Purification

Size

100 µg

Price (USD)

$307

Background

Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the mains, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved (1,2). Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer?s disease including -secretase presenilin and -amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid- . Caspase-12 is ubiquitously expressed in mouse tissues (1).

Immunogen

Synthetic peptide corresponding to amino acids 2 to 17 of the murine caspase-12

Positive Control

Murine spleen tissue lysate

Formulation

Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

38.9 kDa

Product Image

Image Legend

Western blot analysis using anti-Caspase-12 (NT) antibody at 1 µg/ml on human (1) and mouse (2) spleen tissue lysate.

Database Links:

SwissProtQ6UXS9Human

References

1. Nakagawa, T., et al., Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-beta. Nature 2000, 403, 98-103.

2. Mehmet, H., Caspases find a new place to hide. Nature 2000, 403, 29-30

3. Van de Craen M, et al., Characterization of seven murine caspase family members. FEBS Lett 1997, 403, 61-9


Product Specific References


This product has been used in:

1. Hetz, C., et al, 'Caspase-12 and endoplasmic reticulum stress mediate neurotoxicity of pathological prion protein' EMBO Journal 2003, 22, , 5435-54
2. Hetz, C., et al, 'The Disulfide Isomerase Grp58 Is a Protective Factor against Prion Neurotoxicity' Journal of Neuroscience 2005, 25, , 2793-2802
3. Rao, Rammohan V., et al, 'Coupling Endoplasmic Reticulum Stress to the Cell Death Program' The Journal of Biological Chemistry 2002, 277, 24, 21836-21842
4. Moreno, J.A., et al. ?Sustained translational repression by eIF2 ?P mediates prion neurodegeneration.? Nature, 485, 507-511 (2012)
5. Sun, W., et al. ?Magnolia Extract (BL153) Protection of Heart from Lipid Accumulation Caused Cardiac Oxidative Damage, Inflammation, and Cell Death in High-Fat Diet Fed Mice? Oxid. Med. Cell. Longev. (2014), 205849
6. Zhao, Y., et al. ?A Novel Mechanism by Which SDF-1 Protects Cardiac Cells From Palmitate-Induced Endoplasmic Reticulum Stress and Apoptosis via CXCR7 and AMPK/p38 MAPK-Mediated Interleukin-6 Generation? Diabetes (2013), 62, 2545-2558