Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery
Exalpha Biologicals, Inc.

Caspase-12 (IN)

  • Product Code: X1114P
  • Size: 100 µg
  • Price (USD): $383

Cat #

X1114P		 Quantity:      

Data Sheet

Product Name

Caspase-12 (IN)

Host/Source

Rabbit

Isotype

IgG

Product Type

Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purification

Antigen Immunoaffiinity Purification

Size

100 µg

Price (USD)

$383

Background

Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the mains, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved (1,2). Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer’s disease including γ-secretase presenilin and β-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-β. Caspase-12 is ubiquitously expressed in mouse tissues (1).

Immunogen

Synthetic peptide corresponding to amino acids 100 to 116 of the murine caspase-12

Positive Control

Murine spleen tissue lysate

Formulation

Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

38.9 kDa

Product Image

Image Legend

Western blot analysis using anti-Capase-12 (IN) antibody at1 µg/ml on mouse brain tissue in the absense (1) and presense (2) of blocking peptide.

Database Links:

SwissProtQ6UXS9Human

References

1. Nakagawa, T., et al., Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-beta. Nature 2000, 403, 98-103.

2. Mehmet, H., Caspases find a new place to hide. Nature 2000, 403, 29-30

3. Van de Craen M, et al., Characterization of seven murine caspase family members. FEBS Lett 1997, 403, 61-9


Product Specific References


This product has been used in:

1. Hetz, C., et al, 'Caspase-12 and endoplasmic reticulum stress mediate neurotoxicity of pathological prion protein' EMBO Journal 2003, 22, , 5435-54
2. Hetz, C., et al, 'The Disulfide Isomerase Grp58 Is a Protective Factor against Prion Neurotoxicity' Journal of Neuroscience 2005, 25, , 2793-2802
3. Rao, Rammohan V., et al, 'Coupling Endoplasmic Reticulum Stress to the Cell Death Program' The Journal of Biological Chemistry 2002, 277, 24, 21836-21842
4. Moreno, J.A., et al. ‘Sustained translational repression by eIF2α–P mediates prion neurodegeneration.’ Nature, 485, 507-511 (2012)
5. Sun, W., et al. ‘Magnolia Extract (BL153) Protection of Heart from Lipid Accumulation Caused Cardiac Oxidative Damage, Inflammation, and Cell Death in High-Fat Diet Fed Mice’ Oxid. Med. Cell. Longev. (2014), 205849
6. Zhao, Y., et al. ‘A Novel Mechanism by Which SDF-1β Protects Cardiac Cells From Palmitate-Induced Endoplasmic Reticulum Stress and Apoptosis via CXCR7 and AMPK/p38 MAPK-Mediated Interleukin-6 Generation’ Diabetes (2013), 62, 2545-2558