Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Product Highlight

Mouse anti-M13 phage coat protein g8p

Antibodies recognising M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. The monoclonal antibodies manufactured and supplied by Exalpha react with either the pIII (g3p) or pVIII (g8p) proteins of M13 filamentous bacteriophage. All antibodies are available in a purified format. The antibodies are fully validated and are suitable for a wide range of techniques including:

  • ELISA
  • Flow Cytometry
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation
For more information, click here for our M13 Bacteriophage information page.

News

Two more of our excellent products have been published by PubMed:

Potential actionable targets in appendiceal cancer detected by immunohistochemistry, fluorescent in situ hybridization, and mutational analysis
Borazanci, E., et al., J. Gastrointest. Oncol., 8, 164-172 (2017)
Using Exalpha SPARC Antibody (Cat. No. X1867P)

Molecular mechanism underlying the pharmacological interactions of the protein kinase C-β inhibitor enzastaurin and erlotinib in non-small cell lung cancer cells
Steen, N.V., et al., Am. J. Cancer Res., 7, 816-830 (2017)
Using Exalpha's FITC labeled anti PY20 Antibody (Cat. No. X1017)

Exalpha Biologicals, Inc.

Aif (NT) apoptosis inducing factor

  • Product Code: X1109P
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $383

Cat #

X1109P		 Quantity:      

Data Sheet

Product Name

Aif (NT) apoptosis inducing factor

Synonyms

Apoptosis Inducing Factor (NT)

Host/Source

Rabbit

Isotype

IgG

Product Type

Polyclonal Antibody

Reactivity

Human

Applications

Western Blot

Purification

Antigen Immunoaffiinity Purification

Size

100 µg

Price (USD)

$383

Background

Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins (1). A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) (2). Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection. AIF is highly conserved between human and mouse and widely expressed (2).

Immunogen

A synthetic peptide corresponding to amino acids 109 to 122 of human AIF (2). This sequence is identical to those of mouse and rat AIF (2).

Positive Control

K562 cell lysate

Formulation

Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

67

Product Image

Image Legend

Western blot analysis using anti-AIF (NT) antibody at 1 µg/ml on K562 cell lysate.

Database Links:

SwissProtQ9JM53Rat
SwissProtQ9Z0X1Mouse
SwissProtO95831Human

References

1. Condensed matter in cell death. Zamzami, N. and Kroemer, G. Nature 1999, 401, 127-8

2. Molecular characterization of mitochondrial apoptosis-inducing factor. Susin, S.A., et al., Nature 1999, 397, 441-6


Product Specific References


This product has been used in:

1. Pantic, B., et al. ‘Myotonic dystrophy protein kinase (DMPK) prevents ROS-induced cell death by assembling a hexokinase II-Src complex on the mitochondrial surface.’ Cell Death and Disease, Oct 2013, 4(10): e858