Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

Aif (IN) apoptosis inducing factor

  • Product Code: X1108P
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $307

Cat #

X1108P		 Quantity:      

Data Sheet

Product Name

Aif (IN) apoptosis inducing factor

Synonyms

Apoptosis Inducing Factor (IN)

Host/Source

Rabbit

Isotype

IgG

Product Type

Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purification

Antigen Immunoaffiinity Purification

Size

100 µg

Price (USD)

$307

Background

Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins (1). A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) (2). Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection. AIF is highly conserved between human and mouse and widely expressed (2).

Immunogen

A synthetic peptide corresponding to amino acids 517 to 531 of human AIF (2). This sequence is identical to those of mouse and rat AIF (2).

Positive Control

K562 cell lysate

Formulation

Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

67

Product Image

Image Legend

Western blot analysis using anti-AIF (IN) antibody at 1 µg/ml on K562 cell lysate (A) and rat (B) and mouse (C) heart tissue lysates.

Database Links:

SwissProtQ9JM53Rat
SwissProtQ9Z0X1Mouse
SwissProtO95831Human

References

1. Condensed matter in cell death. Zamzami, N. and Kroemer, G. Nature 1999, 401, 127-8

2. Molecular characterization of mitochondrial apoptosis-inducing factor. Susin, S.A., et al., Nature 1999, 397, 441-6


Product Specific References


This product has been used in:

1. Pantic, B., et al. ?Myotonic dystrophy protein kinase (DMPK) prevents ROS-induced cell death by assembling a hexokinase II-Src complex on the mitochondrial surface.? Cell Death and Disease, Oct 2013, 4(10): e858