Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Exalpha Biologicals, Inc.

ADAM 10 (CT) (kuz) (TNFa converting enzyme)

  • Product Code: X1106P
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $307

Cat #

X1106P		 Quantity:      

Data Sheet

Product Name

ADAM 10 (CT) (kuz) (TNFa converting enzyme)

Synonyms

KUZ; MADM

Host/Source

Rabbit

Isotype

IgG

Product Type

Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purification

Antigen Immunoaffiinity Purification

Size

100 µg

Price (USD)

$307

Background

Proinflammatory cytokine tumor necrosis factor- (TNF- ) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF- , notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF- , notch and its ligand delta (1-3). The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively, (1,2,4). ADAM10 mRNA is expressed in a variety of human and bovine tissues (1,4).

Immunogen

Synthetic peptide corresponding to amino acids 732 to 748 off the human ADAM10. Immungen sequence is identical in bovine and rat and differs from the mouse protein by one amino acid.

Positive Control

Jurkat whole cell lysate

Formulation

Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

85 kDa

Product Image

Image Legend

Western blot analysis using anti-ADAM10 (CT) antibody at 0.5 µg/ml on Jurkat cell lysate for ADAM10. 1) No blocking peptide used. 2) Blocking peptide used.

Database Links:

SwissProtQ10743Rat
SwissProtO35598Mouse
SwissProtO14672Human

References

1. Rosendahl, M.S., et al., Identification and characterization of a pro-tumor necrosis factor-alpha-processing enzyme from the ADAM family of zinc metalloproteases. J. Biol. Chem. 1997, 272, 24588-24593

2. Pan, D. & Rubin, G.M., Kuzbanian controls proteolytic processing of Notch and mediates lateral inhibition during Drosophila and vertebrate neurogenesis. Cell 1997, 90, 271-280

3. Qi, H., et al., Processing of the notch ligand delta by the metalloprotease Kuzbanian. Science 1999, 283, 91-94

4. Howard, L., et al., Molecular cloning of MADM: a catalytically active mammalian disintegrin-metalloprotease expressed in various cell types. Biochem. J. 1996, 317, 45-50