Exalpha Biologicals, Inc.

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Exalpha Biologicals, Inc. is now a US distributor for Nordic-Mubio products

Nordic-MUbio develops and manufactures high quality antibody reagents for use in R&D. All our products are manufactured according to strict ISO 9001 quality guidelines, and our focus is on developing antibodies targetting Cell Adhesion, Nuclear and Cytoskeletal Proteins.

Nordic-MUbio is one of the leading antibody companies developing antibodies for zebrafish research – we currently have over 50 antibodies validated for immunofluorescence in zebrafish.

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Latest References

Akazawa, Y., et al. "Endoplasmic Reticulum Stress Contributes to Helicobacter Pylori VacA-Induced Apoptosis" PLoS ONE 8(12): e82322

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Homa, N.J., et al. "Epstein-Barr Virus Induces Global Changes in Cellular mRNA Isoform Usage That Are Important for the Maintenance of Latency" J. Virol., 2013, 87, 12291-12301

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Exalpha Biologicals, Inc.

Acinus (NP)

  • Product Code: X1105P
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $307

Cat #

X1105P		 Quantity:      

Data Sheet

Product Name

Acinus (NP)

Synonyms

KIAA0670

Host/Source

Rabbit

Isotype

IgG

Product Type

Polyclonal Antibody

Reactivity

Human

Applications

Western Blot

Purification

Antigen Immunoaffiinity Purification

Size

100 µg

Price (USD)

$307

Background

Chromatin condensation and nuclear fragmentation (CCNF) is the hallmark of apoptosis. CCNF is triggered by the activation of members of caspase family, caspase activated DNase (CAD/DFF40), and several novel proteins including AIF and CIDE (1). A new inducer of chromatin condensation was recently identified and designated Acinus (for apoptotic chromatin condensation inducer in the nucleus). Acinus is cleaved by caspase-3 and an additional unknown protease generating a small active peptide p17, which causes chromatin condensation in vitro when it is added to purified nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and designated AcinusL, AcinusS and AcinusS? (2).

Immunogen

Synthetic peptide (DDPVRTAQVPSPPRGK) corresponding to amino acids 994 to 1009 of human AcinusL, 267 to 282 of human AcinusS?, or 236 to 251 of human AcinusS, which are identical to those of mouse Acinus (2). The selected antigenic sequence is located near the N-terminus of active peptide p17.

Positive Control

K562 cell lysate

Formulation

Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

220

Product Image

Image Legend

Western blot analysis using anti-Acinus (NP) antibody at 1 µg/ml on K562 cell lysate.

Database Links:

SwissProtQ9UKV3Human

References

1. Condensed matter in cell death. Zamzami, N and Kroemer, G. , Nature, 1999 , 401, 127-8

2. Acinus is a caspase-3-activated protein required for apoptotic chromatin condensation. Sahara S, et al., Nature, 1999, 401, 168-73