Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery
Exalpha Biologicals, Inc.

Bovine Type I Collagen Substrate, FITC Conjugate

  • Product Code: X1098
  • Size: 10 mg
  • Price (USD): $361

Cat #

X1098		 Quantity:      

Data Sheet

Product Name

Bovine Type I Collagen Substrate, FITC Conjugate

Synonyms

Extracted from bovine skin

Host/Source

Bovine

Clone

N/A

Isotype

N/A

Product Type

Purified Protein

Applications

Substrate

Size

10 mg

Price (USD)

$361

Background

FITC-labeled bovine type I Collagen Substrate is an excellent substrate for examining collagenase activity. This labelled substrate is highly purified and telo-peptide free and is supplied lyophilized. To minimize background levels in collagenase assays, FITC-labeled collagen has been enzymatically pre-treated and further purified by ion-exchange chromatography. FITC-labeled collagen can also be used as a substrate for cell culture. Collagen degradation products in the culture supernatants can be directly determined by measured the flourescence at 520 nm (Emission)/490 nm (Excitation).

Immunogen

Bovine skin

Formulation

Provided as a 1 mg/ml solution in 0.01M acetic acid.

Customer Storage

Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

References

1. Terato, K., et al. 'A rapid assay method of collagenase activity using 14C-labeled soluble collagen as substrate.' Biochim. Biophys. Acta 1976, 445, 753-762 2. Sellers, A., et al. 'Evidence that latent collagenases are enzyme-inhibitor complexes.' Biochem. J. 1977, 163, 303-307 3. Shinkai, H., et al. 'A complex of collagenase with low molecular weight inhibitors in the culture medium of embryonic chick skin explants.' J. Biochem. (Tokyo) 1977, 81, 261-263 4. Shinkai, H. & Nagai, Y., 'A latent collagenase from embryonic human skin explants.' J. Biochem. (Tokyo) 1977, 81, 1261-1263 5. Murawaki, Y., et al. 'Serum collagenase activity in patients with chronic liver disease.' J. Hepatol. 1993, 328-334