Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Exalpha Biologicals, Inc.

Sphingosine 1 Phosphate Receptor 5 CT (EDG-8)

  • Product Code: X1094P
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $438

Cat #

X1094P		 Quantity:      

Data Sheet

Product Name

Sphingosine 1 Phosphate Receptor 5 CT (EDG-8)

Synonyms

Endothelial Cell Differentiation Gene-8 Receptor C-Terminal

Host/Source

Rabbit

Isotype

IgG

Product Type

Polyclonal Antibody

Reactivity

Human

Applications

Western Blot

Purification

Ammonium Sulfate Precipitation

Size

100 µg

Price (USD)

$438

Background

Endothelial Cell Differentiation Gene-8 (EDG-8) belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG-8 is sphingosine-1-phosphate. There are 8 known members of the EDG receptor family and they are implicated in mediating growth related effects such as induction of cellular proliferation, alterations in differentiation and survival and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG-8 is expressed in oligodendrocytes and fibrous astrocytes in the rat brain.

Immunogen

Unique synthetic peptide derived from the C-terminus of the human EDG-8 protein

Positive Control

RH7777 cells transfected with full length EDG8 protein.

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

42

Product Image

Image Legend

Western blot analysis using anti-EDG-8 CT antibody on RH7777 cell lysates transfected with full length human EDG8 (1) and blocked with blocking peptide (2) using Pierce Femto Signal substrate.

Database Links:

SwissProtQ9H228Human

References

1. Im, D.S., et al. 'Characterization of a novel sphingosine-1-phosphate receptor, Edg-8.' J. Biol. Chem. 2000, 275, 14281-14286
2. Malek, R.L., et al. Nrg-1 belongs to the EDG family of G-protein coupled sphingosine-1-phosphate receptors. J. Biol. Chem. 2000, epub ahead of print.
3. Hla, T., et al. Sphingosine-1-phosphate signaling via the EDG-1 family of G-protein coupled receptors. Ann. N.Y. Acad. Sci. 2000, 905, 16-24
4. Pyne, S. & Pyne, N.J. Sphingosine-1-phosphate signalling in mammalian cells. Biochem. J. 2000, 349, 385-402


Product Specific References


This product has been used in:

1. Toman, Rachelle E. et al, 'Differential transactivation of sphingosine-1-phosphate receptors modulates NGF-induced neurite extesion' The Journal of Cell Biology 2004, 166, 3, 381-392