Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery
Exalpha Biologicals, Inc.

Sphingosine 1 Phosphate Receptor 5 CT (EDG-8)

  • Product Code: X1094P
  • Size: 100 µg
  • Price (USD): $438

Cat #

X1094P		 Quantity:      

Data Sheet

Product Name

Sphingosine 1 Phosphate Receptor 5 CT (EDG-8)

Synonyms

Endothelial Cell Differentiation Gene-8 Receptor C-Terminal

Host/Source

Rabbit

Isotype

IgG

Product Type

Polyclonal Antibody

Reactivity

Human

Applications

Western Blot

Purification

Ammonium Sulfate Precipitation

Size

100 µg

Price (USD)

$438

Background

Endothelial Cell Differentiation Gene-8 (EDG-8) belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG-8 is sphingosine-1-phosphate. There are 8 known members of the EDG receptor family and they are implicated in mediating growth related effects such as induction of cellular proliferation, alterations in differentiation and survival and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG-8 is expressed in oligodendrocytes and fibrous astrocytes in the rat brain.

Immunogen

Unique synthetic peptide derived from the C-terminus of the human EDG-8 protein

Positive Control

RH7777 cells transfected with full length EDG8 protein.

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

42

Product Image

Image Legend

Western blot analysis using anti-EDG-8 CT antibody on RH7777 cell lysates transfected with full length human EDG8 (1) and blocked with blocking peptide (2) using Pierce Femto Signal substrate.

Database Links:

SwissProtQ9H228Human

References

1. Im, D.S., et al. 'Characterization of a novel sphingosine-1-phosphate receptor, Edg-8.' J. Biol. Chem. 2000, 275, 14281-14286 2. Malek, R.L., et al. Nrg-1 belongs to the EDG family of G-protein coupled sphingosine-1-phosphate receptors. J. Biol. Chem. 2000, epub ahead of print. 3. Hla, T., et al. Sphingosine-1-phosphate signaling via the EDG-1 family of G-protein coupled receptors. Ann. N.Y. Acad. Sci. 2000, 905, 16-24 4. Pyne, S. & Pyne, N.J. Sphingosine-1-phosphate signalling in mammalian cells. Biochem. J. 2000, 349, 385-402


Product Specific References


This product has been used in:

1. Toman, Rachelle E. et al, 'Differential transactivation of sphingosine-1-phosphate receptors modulates NGF-induced neurite extesion' The Journal of Cell Biology 2004, 166, 3, 381-392