Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Exalpha Biologicals, Inc.

Sphingosine 1 Phosphate Receptor 1 (EDG-1) CT

  • Product Code: X1093P
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $438

Cat #

X1093P		 Quantity:      

Data Sheet

Product Name

Sphingosine 1 Phosphate Receptor 1 (EDG-1) CT

Synonyms

Endothelial cell differentiation gene 1 C-terminal

Host/Source

Rabbit

Isotype

IgG

Product Type

Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purification

Ammonium Sulfate Precipitation

Size

100 µg

Price (USD)

$438

Background

EDG-1 belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG-1 is sphingosine-1-phosphate. There are 8 known members of the EDG receptor family and they are implicated in mediating growth related effects such as induction of cellular proliferation, alterations in differentiation and survival and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG-1 is expressed in cardiovascular, leukocyte- containing and other tissues.

Immunogen

Synthetic peptide derived from the C-terminal of the EDG-1 receptor

Positive Control

RH7777 cells transfected with full length EDG-1 protein.

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

44

Product Image

Image Legend

Western blot analysis of lysates of RH7777 cells transfected with full lenth human EDG-1 protein using anti-EDG-1 CT antibody at 10 µg/ml (1), antibody preincubated with specific blocking peptide (2) and antibody preincubated with non-specific control peptide (3) using Pierce Femto Signal substrate.

Database Links:

SwissProtP48303Rat
SwissProtO08530Mouse
SwissProtP21453Human

References

1. Edg-1, the G protein-coupled receptor for sphingosine-1-phosphate, is essential for vascular maturation. Liu, Y., et al., J. Clin. Invest., 2000, 106, 951-961.

2. Expression and characterization of edg-1 receptors in rat cardiomyocytes calcium deregulation in response to sphingosine-1-phosphate. Nakajima, N., et al., Eur. J. Biochem., 2000, 267, 5679-5686.

3. Diversity of cellular receptors and functions for the lysophospholipid growth factors lysophosphatidic acid and sphingosine 1-phosphate. Goetzl, E.J. and An, S.,FASEB J., 12(15), 1589-1598 (1998).



Product Specific References


This product has been used in:

1) Western Blot: 'Transduction of Multiple Effects of Sphingosine 1-Phosphate (S1P) on T Cell Function by the S1P1 G Protein Coupled Receptor.' Dorsam, Glenn, et al., Journal of Immunology, 2003, 3500-3507.
2) Immunocytochemistry: 'Differential transactivation of Sphingoise-1 Phosphate Receptors modulate NGF-induced neurite extension.' Toman, Rachelle E., et al., Journal of Cell Biology, Aug. 2, 2004, vol 166, no.3, 381-392.
3) Western Blot: 'Transactivation of Sphingosine-1-Phosphate Recpetors by FceRI Triggering is Required for Normal Mast Cell Degranulation and Chemotaxis.' Jolly, Puneet S., et al., Journal of Experimental Medicine, April 5, 2004, vol. 199, no. 7, 959-970.
4) Western Blot: 'Induction of pro-angiogenic signaling by a synthetic peptide derived from the second intracellular loop of S1P3 (EDG3).' Blood, 2003, 102, 2099-2107.