Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

3BP2 (SH3 Binding Protein 2)

  • Product Code: X1090P
  • Size: 250 µg
  • Availability: In Stock In Stock
  • Price (USD): $244

Cat #

X1090P		 Quantity:      

Data Sheet

Product Name

3BP2 (SH3 Binding Protein 2)

Synonyms

Sh3bp2, SH3 domain-binding protein 2

Host/Source

Sheep

Isotype

IgG

Product Type

Polyclonal Antibody

Reactivity

Mouse

Applications

Western Blot

Purification

Ammonium Sulfate Precipitation

Size

250 µg

Price (USD)

$244

Background

3BP2 (SH3 binding protein 2) was identified as a protein binding to the SH3 domain of the Abl proto-oncogene. Murine 3BP2 cDNA encodes a 559 amino acid protein of 85 kDa consisting of an N-terminal PH domain, a C-terminal SH2 domain and intervening proline-rich sequences. 3BP2 interacts via its Sh2 domain with Syk family protein tyrosine kinases Syk, Zap70, Grb2, Cbl, PLC 1 and LAT in T lymphocytes and contributes to the transcriptional activation of NF-AT and AP-1. Mouse 3BP2 is 66% homologous to the human protein at the amino acid level.

Immunogen

Antibody developed using SH2 domain of the 3BP2 protein fused to GST.

Positive Control

Recombinant 3BP2 protein

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

85

Product Image

Image Legend

Western blot analysis using anti-3BP2 at 10 µg/ml on recombinant full lenth 3BP2 protein (1), 3BP2 protein minus the PH domain (2), 3BP2 protein minus the PR domain (3) and 3BP2 protein minus the SH2 domain (4).

Database Links:

SwissProtQ06649Human

References

1. Decker, M., et al. Adaptor function for the Syk kinases-interacting protein 3BP2 in IL-2 gene activation. Immunity 1998, 9, 595-605

2. Songyang, Z., et al. Specific motifs recognized by the SH2 domains of Csk, 3BP2, fps/fes, GRB-2, HCP, SHC, Syk and Vav. Mol. Cell Biol. 1994, 14, 2777-2785