Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Exalpha Biologicals, Inc.

TCF-1

  • Product Code: X1072M
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $407

Cat #

X1072M		 Quantity:      

Data Sheet

Product Name

TCF-1

Synonyms

Anti TCF-1 Monoclonal Antibody, Clone 7H3, T Cell Factor 1

Host/Source

Mouse

Clone

7H3

Isotype

IgG

Product Type

Monoclonal Antibody

Reactivity

Human

Applications

Western Blot, Immunohistochemistry (Frozen Sections)

Purification

Protein A/G Chromatography

Size

100 µg

Price (USD)

$407

Background

TCF 1 (T cell factor 1) is a member of the TCF/LEF (lymphoid enhancer factor) family of transcription factors. Members of this family along with catenin, are involved in mediating Wnt signaling by transactivating downstream genes. TCF 1 is closely related to LEF-1, both of which are transcription factors expressed in mice during T cell differentiation. They regulate the T cell receptor enhancer in transfections assays. TCF 1 becomes transcriptionally active through interaction with catenin. Also, numerous splice variants of TCF 1 have been identified. Overexpression of TCF-1-specific splice forms appears to correlate with the metastatic behavior of colorectal cancer cells and with overproduction of lymphoid tyrosine protein kinase p56(lck).

Immunogen

Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant protein corresponding to amino acids 102-269 from the human TCF1A splice variant protein fused to maltose binding protein (MBP)

Positive Control

Specificity: Recognizes multiple splice isoforms of TCF-1.

Formulation

Provided as 0.2µm sterile filtered solution in 0.1 M tris-glycine, pH 7.4, 0.150M NaCl, 30 % glycerol with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Image

Image Legend

Western blot analysis using anti-TCF-1 clone 7H3 antibody at 1 µg/ml on Jurkat cell lysates.

Database Links:

SwissProtP36402Human

References

1. Staal, F.J., et al. Tcf-1-mediated transcription in T lymphocytes: differential role for glycogen synthase kinase-3 in fibroblasts and T cells. Int. Immunol. 1999, 11, 317-323

2. Mayer, K., et al. Ectopic activation of lymphoid high mobility group-box transcription factor TCF-1 and overexpression in colorectal cancer cells. Int. J. Cancer 1997, 72, 625-630

3. Castrop, K., et al. The human TCF-1 gene encodes a nuclear DNA-binding protein uniquely expressed in normal and neoplastic T-linkage lymphocytes. Blood 1995, 86, 3050-3059

4. Van de Wetering, M, et al. 'Extensive alternative splicing and dual promoter usage generate Tcf-1 protein isoforms with differential transcription control properties.' Mol. Cell. Biol., 16: 745-52 (1996).

Product Specific References:

1. Lin, C-W, et al. 'CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features.' Blood, 2005, 106, 3567-3574.


Product Specific References


This product has been used in:

1. Dorfman, David M. et al., 'Loss of Expression of the WNT/Beta Catenin Signaling Pathway Transcription Factors Lymphoid Enhancer Factor-1 (LEF-1) and T Cell factor-1 (TCF-10 in a Subset of Peripheral T Cel Lymphomas' The American Journal of Pathology 2003, 162, , 1539-1544
2. Tobimatsu, Takako, et al, 'Parathyroid Hormone Increaes Beta Catenin levels through Smad3 in Mouse Osteblastic Cells' Endocrinology 2006, 147, 5, 2583-2590