Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery
Exalpha Biologicals, Inc.

TCF-3/4

  • Product Code: X1071M
  • Size: 100 µg
  • Price (USD): $283

Cat #

X1071M		 Quantity:      

Product Name

TCF-3/4

Synonyms

T-Cell Factor-3/4

Host/Source

Mouse

Clone

6F12.3

Isotype

IgG1

Product Type

Monoclonal Antibody

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry (Paraffin Sections), Gel Supershift

Purification

Protein A/G Chromatography

Size

100 µg

Price (USD)

$283

Background

TCF-3 and TCF-4 (T cell factor-4) are a members of the TCF/LEF (lymphoid enhancer factor) family of transcription factors. Members of this family along with ? catenin, are involved in mediating Wnt signaling by transactivating downstream genes. TCF-4 generates transcriptionally active complexes with ? catenin in colon carcinoma cells. Expression of TCF-4 is highest in the CNS and intestinal epithelium where Wnt signaling also plays an essential role in development. Aberrant TCF-4 transcription activity has been implicated in carcinogenesis of intestinal and mammary epithlia. TCF-3 appears to be expressed exclusively of TCF-4 in the hair follicles, stomach epithelium, and keratinocytes of the skin.

Immunogen

His-tagged fusion protein corresponding to amino acids 31-331 of the human TCF

Positive Control

Specificity: TCF-3 and TCF-4

Formulation

Provided as 0.2µm sterile filtered solution in 0.1 M tris-glycine, pH 7.4, 0.150M NaCl, 30 % glycerol with 0.08% sodium azide

Customer Storage

Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

60

Product Image

Image Legend

Western blot analysis using anti-TCF-3/4 clone 6F12-3 antibody at 4 µg/ml on Cos-1 cell control lysates (1) and Cos-1 cell lysates transfected with human TCF-3.

Database Links:

SwissProtQ924A0Mouse
SwissProtQ9NQB0Human

References

1. Barker, N., et al., 'Restricted high level expression of Tcf-4 protein in intestinal and mammary gland epithelium. Am. J. Pathol. 1999 154, 29-35 2. Pollheime, J., et al. 'Activation of the Canonical Wingless/T-Cell Factor Signaling Pathway Promotes Invasive Differentiation of Human Trophoblast.' Am. J. Pathol. 2006, 168, 1134-1147. 3. Palacino, J J, et al. 'Presenilin 1 regulates beta-catenin-mediated transcription in a glycogen synthase kinase-3-independent fashion.' J. Biol. Chem., 276: 38563-9 (2001) 4. Amir, A L., et al. 'A Direct {beta}-Catenin-independent Interaction between Androgen Receptor and T Cell Factor 4.' J Biol Chem, 278:30828-30834 (2003)


Product Specific References


This product has been used in:

1. Lee. Ethan, et al., 'Physiological regulation of Beta-Catenin stability by TCF3 and cK1 epsilon' The Jouranl of Cell Biology 2001, 154, 5, 983-994 2. Denys, H., et al, 'Identification of IGFBP-6 as a significantly downregulated gene by -catenin in desmoid tumors' Oncogene 2004, 23, , 654-664 3. Liu, G., et al. 'N-Glycosylation Induces the CTHRC1 Protein and Drives Oral Cancer Cell Migration.' The Journal of Biological Chemistry, Jul 2013, 288(28): 20217-20227. 4. Sengupta, P.K., et al. ' Coordinate regulation of N-glycosylation gene DPAGT1, canonical Wnt signaling and E-cadherin adhesion.' Journal of Cell Science, Jan 2013, 126(2): 484-496.