Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Product Highlight

Mouse anti-M13 phage coat protein g8p

Antibodies recognising M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. The monoclonal antibodies manufactured and supplied by Exalpha react with either the pIII (g3p) or pVIII (g8p) proteins of M13 filamentous bacteriophage. All antibodies are available in a purified format. The antibodies are fully validated and are suitable for a wide range of techniques including:

  • ELISA
  • Flow Cytometry
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation
For more information, click here for our M13 Bacteriophage information page.

News

Two more of our excellent products have been published by PubMed:

Potential actionable targets in appendiceal cancer detected by immunohistochemistry, fluorescent in situ hybridization, and mutational analysis
Borazanci, E., et al., J. Gastrointest. Oncol., 8, 164-172 (2017)
Using Exalpha SPARC Antibody (Cat. No. X1867P)

Molecular mechanism underlying the pharmacological interactions of the protein kinase C-β inhibitor enzastaurin and erlotinib in non-small cell lung cancer cells
Steen, N.V., et al., Am. J. Cancer Res., 7, 816-830 (2017)
Using Exalpha's FITC labeled anti PY20 Antibody (Cat. No. X1017)

Exalpha Biologicals, Inc.

TCF-4

  • Product Code: X1070M
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $316

Cat #

X1070M		 Quantity:      

Data Sheet

Product Name

TCF-4

Synonyms

T-Cell Factor-4, BHLHB19, ITF2, SEF2

Host/Source

Mouse

Clone

6H5.3

Isotype

IgG2a

Product Type

Monoclonal Antibody

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunoprecipitation

Purification

Protein A/G Chromatography

Size

100 µg

Price (USD)

$316

Background

TCF 4 (T cell factor 4) is a member of the TCF/LEF (lymphoid enhancer factor) family of transcription factors. Members of this family along with β catenin, are involved in mediating Wnt signaling by transactivating downstream genes. TCF 4 generates transcriptionally active complexes with β catenin in colon carcinoma cells. Expression of TCF 4 is highest in the CNS and intestinal epithelium where Wnt signaling also plays an essential role in development. Aberrant TCF 4 transcription activity has been implicated in carcinogenesis of intestinal and mammary epithlia.

Immunogen

Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant protein corresponding to amino acids 31-331 of the human TCF-4 protein.

Positive Control

Cos-1 cells, HCT116 cells

Formulation

Provided as 0.2µm sterile filtered solution in 0.7 M tris-glycine, pH 7.4, 0.105M NaCl, 30% glycerol with 0.035% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

66

Product Image

Image Legend

Western blot analysis using anti-TCF-4, clone 6H5-3 antibody at 1 µg/ml on Cos-1 cell lysate control (1) and Cos-1 cells transfected with human TCF-4 (2).

Database Links:

SwissProtQ60722Mouse
SwissProtP15884Human

References

1. Barker, N., et al. Resticted high level expression of TCF-4 protein in intestinal and mammary gland epithelium. Am. J. Pathol. 1999, 154, 29-35

2. Ishitani, M.L., et al. The TAK1-NLK-MAPK-related pathway antagonizes signalling between beta-catenin and transcription factor TCF. Nature 1999, 399, 798-802

3. Korinek, V., et al. Depletion of epithelial stem-cell compartments in the small intestine of mice lacking Tcf-4. Nature Genetics 1998, 19, 379-383


Product Specific References


This product has been used in:

1. Sampson, Ellen, M., et al, 'Negative regulation of the Wnt- Beta-Catenin pathway by the transcriptional represor HBPQ' The EMBO Journal 2001, 20, , 4500-4511
2. Uematsu, Kazutsugu, et al, 'Wnt Pathway Activation in Mesothelioma: Evidence of Dishevelled Overexpression and Transcriptional Activity of Beta Catenin' Journal of Cancer Research 2003, 63, , 4547-4551
3. Shimura, Tatsuo, 'Galectin-3, a Novel Binding Partner of Beta Catenin' Cancer Research 2004, 64, , 6363-6367