Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery
Exalpha Biologicals, Inc.

TCF-4

  • Product Code: X1070M
  • Size: 100 µg
  • Price (USD): $316

Cat #

X1070M		 Quantity:      

Data Sheet

Product Name

TCF-4

Synonyms

T-Cell Factor-4, BHLHB19, ITF2, SEF2

Host/Source

Mouse

Clone

6H5.3

Isotype

IgG2a

Product Type

Monoclonal Antibody

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunoprecipitation

Purification

Protein A/G Chromatography

Size

100 µg

Price (USD)

$316

Background

TCF 4 (T cell factor 4) is a member of the TCF/LEF (lymphoid enhancer factor) family of transcription factors. Members of this family along with ? catenin, are involved in mediating Wnt signaling by transactivating downstream genes. TCF 4 generates transcriptionally active complexes with ? catenin in colon carcinoma cells. Expression of TCF 4 is highest in the CNS and intestinal epithelium where Wnt signaling also plays an essential role in development. Aberrant TCF 4 transcription activity has been implicated in carcinogenesis of intestinal and mammary epithlia.

Immunogen

Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant protein corresponding to amino acids 31-331 of the human TCF-4 protein.

Positive Control

Cos-1 cells, HCT116 cells

Formulation

Provided as 0.2µm sterile filtered solution in 0.7 M tris-glycine, pH 7.4, 0.105M NaCl, 30% glycerol with 0.035% sodium azide

Customer Storage

Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

66

Product Image

Image Legend

Western blot analysis using anti-TCF-4, clone 6H5-3 antibody at 1 µg/ml on Cos-1 cell lysate control (1) and Cos-1 cells transfected with human TCF-4 (2).

Database Links:

SwissProtQ60722Mouse
SwissProtP15884Human

References

1. Barker, N., et al. Resticted high level expression of TCF-4 protein in intestinal and mammary gland epithelium. Am. J. Pathol. 1999, 154, 29-35 2. Ishitani, M.L., et al. The TAK1-NLK-MAPK-related pathway antagonizes signalling between beta-catenin and transcription factor TCF. Nature 1999, 399, 798-802 3. Korinek, V., et al. Depletion of epithelial stem-cell compartments in the small intestine of mice lacking Tcf-4. Nature Genetics 1998, 19, 379-383


Product Specific References


This product has been used in:

1. Sampson, Ellen, M., et al, 'Negative regulation of the Wnt- Beta-Catenin pathway by the transcriptional represor HBPQ' The EMBO Journal 2001, 20, , 4500-4511 2. Uematsu, Kazutsugu, et al, 'Wnt Pathway Activation in Mesothelioma: Evidence of Dishevelled Overexpression and Transcriptional Activity of Beta Catenin' Journal of Cancer Research 2003, 63, , 4547-4551 3. Shimura, Tatsuo, 'Galectin-3, a Novel Binding Partner of Beta Catenin' Cancer Research 2004, 64, , 6363-6367