Exalpha Biologicals, Inc.

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

News

Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

TCF-4

  • Product Code: X1070M
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $316

Cat #

X1070M		 Quantity:      

Data Sheet

Product Name

TCF-4

Synonyms

T-Cell Factor-4, BHLHB19, ITF2, SEF2

Host/Source

Mouse

Clone

6H5.3

Isotype

IgG2a

Product Type

Monoclonal Antibody

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunoprecipitation

Purification

Protein A/G Chromatography

Size

100 µg

Price (USD)

$316

Background

TCF 4 (T cell factor 4) is a member of the TCF/LEF (lymphoid enhancer factor) family of transcription factors. Members of this family along with catenin, are involved in mediating Wnt signaling by transactivating downstream genes. TCF 4 generates transcriptionally active complexes with catenin in colon carcinoma cells. Expression of TCF 4 is highest in the CNS and intestinal epithelium where Wnt signaling also plays an essential role in development. Aberrant TCF 4 transcription activity has been implicated in carcinogenesis of intestinal and mammary epithlia.

Immunogen

Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant protein corresponding to amino acids 31-331 of the human TCF-4 protein.

Positive Control

Cos-1 cells, HCT116 cells

Formulation

Provided as 0.2µm sterile filtered solution in 0.7 M tris-glycine, pH 7.4, 0.105M NaCl, 30% glycerol with 0.035% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

66

Product Image

Image Legend

Western blot analysis using anti-TCF-4, clone 6H5-3 antibody at 1 µg/ml on Cos-1 cell lysate control (1) and Cos-1 cells transfected with human TCF-4 (2).

Database Links:

SwissProtQ60722Mouse
SwissProtP15884Human

References

1. Barker, N., et al. Resticted high level expression of TCF-4 protein in intestinal and mammary gland epithelium. Am. J. Pathol. 1999, 154, 29-35

2. Ishitani, M.L., et al. The TAK1-NLK-MAPK-related pathway antagonizes signalling between beta-catenin and transcription factor TCF. Nature 1999, 399, 798-802

3. Korinek, V., et al. Depletion of epithelial stem-cell compartments in the small intestine of mice lacking Tcf-4. Nature Genetics 1998, 19, 379-383


Product Specific References


This product has been used in:

1. Sampson, Ellen, M., et al, 'Negative regulation of the Wnt- Beta-Catenin pathway by the transcriptional represor HBPQ' The EMBO Journal 2001, 20, , 4500-4511
2. Uematsu, Kazutsugu, et al, 'Wnt Pathway Activation in Mesothelioma: Evidence of Dishevelled Overexpression and Transcriptional Activity of Beta Catenin' Journal of Cancer Research 2003, 63, , 4547-4551
3. Shimura, Tatsuo, 'Galectin-3, a Novel Binding Partner of Beta Catenin' Cancer Research 2004, 64, , 6363-6367